Oral administration of attenuated S. typhimurium carrying shRNA-expressing vectors as a cancer therapeutic
RNAi has been successfully applied in genomic research, and it also holds considerable promise as a therapeutic approach to suppress disease-causing gene expression. Here, we show that attenuated S. typhimurium were capable of delivering shRNA-expressing vectors to mammalian cells and inducing RNAi...
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| Published in | Cancer biology & therapy Vol. 7; no. 1; pp. 145 - 151 |
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| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
Taylor & Francis
01.01.2008
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| Subjects | |
| Online Access | Get full text |
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| Abstract | RNAi has been successfully applied in genomic research, and it also holds considerable promise as a therapeutic approach to suppress disease-causing gene expression. Here, we show that attenuated S. typhimurium were capable of delivering shRNA-expressing vectors to mammalian cells and inducing RNAi in vitro and in vivo. Upon oral administration, S.typhimurium carrying shRNA-expressing vectors targeting bcl2 induced significant gene silencing in murine melanoma cells that led to a remarkably delayed tumor growth and prolonged survival in the mouse model. These results suggest that bacteria mediated RNAi may be a new potent approach to the treatment of cancers. |
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| AbstractList | RNAi has been successfully applied in genomic research, and it also holds considerable promise as a therapeutic approach to suppress disease-causing gene expression. Here, we show that attenuated S. typhimurium were capable of delivering shRNA-expressing vectors to mammalian cells and inducing RNAi in vitro and in vivo. Upon oral administration, S.typhimurium carrying shRNA-expressing vectors targeting bcl2 induced significant gene silencing in murine melanoma cells that led to a remarkably delayed tumor growth and prolonged survival in the mouse model. These results suggest that bacteria mediated RNAi may be a new potent approach to the treatment of cancers. |
| Author | Chen, Lishan Ren, Daming Li, Shenghua Yang, Nan Zhu, Xiangying |
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| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/18059172$$D View this record in MEDLINE/PubMed |
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| SubjectTerms | Administration, Oral Animals Binding Biology Bioscience Calcium Cancer Cell Cycle Down-Regulation Gene Transfer Techniques Genes, bcl-2 Genetic Vectors Landes Mice Neoplasms - therapy Organogenesis Polymerase Chain Reaction Proteins RNA Interference RNA, Small Interfering - genetics Salmonella typhimurium - genetics |
| Title | Oral administration of attenuated S. typhimurium carrying shRNA-expressing vectors as a cancer therapeutic |
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