Oral administration of attenuated S. typhimurium carrying shRNA-expressing vectors as a cancer therapeutic

RNAi has been successfully applied in genomic research, and it also holds considerable promise as a therapeutic approach to suppress disease-causing gene expression. Here, we show that attenuated S. typhimurium were capable of delivering shRNA-expressing vectors to mammalian cells and inducing RNAi...

Full description

Saved in:
Bibliographic Details
Published inCancer biology & therapy Vol. 7; no. 1; pp. 145 - 151
Main Authors Yang, Nan, Zhu, Xiangying, Chen, Lishan, Li, Shenghua, Ren, Daming
Format Journal Article
LanguageEnglish
Published United States Taylor & Francis 01.01.2008
Subjects
Administration, Oral
Animals
Binding
Biology
Bioscience
Calcium
Cancer
Cell
Cycle
Down-Regulation
Gene Transfer Techniques
Genes, bcl-2
Genetic Vectors
Landes
Mice
Neoplasms - therapy
Organogenesis
Polymerase Chain Reaction
Proteins
RNA Interference
RNA, Small Interfering - genetics
Salmonella typhimurium - genetics
Online AccessGet full text

Cover

More Information
Summary:RNAi has been successfully applied in genomic research, and it also holds considerable promise as a therapeutic approach to suppress disease-causing gene expression. Here, we show that attenuated S. typhimurium were capable of delivering shRNA-expressing vectors to mammalian cells and inducing RNAi in vitro and in vivo. Upon oral administration, S.typhimurium carrying shRNA-expressing vectors targeting bcl2 induced significant gene silencing in murine melanoma cells that led to a remarkably delayed tumor growth and prolonged survival in the mouse model. These results suggest that bacteria mediated RNAi may be a new potent approach to the treatment of cancers.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1538-4047
1555-8576
DOI:10.4161/cbt.7.1.5195