Immune defects in patients with pulmonary Mycobacterium abscessus disease without cystic fibrosis
The prevalence of Mycobacterium abscessus infections in non-cystic fibrosis (CF) patients has increased in recent years. In this study, we investigate whether immune defects explain the apparent susceptibility to this opportunistic infection in non-CF patients. We performed stimulations of periphera...
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Published in | ERJ open research Vol. 6; no. 4; p. 590 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
European Respiratory Society
01.10.2020
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Subjects | |
Online Access | Get full text |
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Summary: | The prevalence of
Mycobacterium abscessus
infections in non-cystic fibrosis (CF) patients has increased in recent years. In this study, we investigate whether immune defects explain the apparent susceptibility to this opportunistic infection in non-CF patients.
We performed stimulations of peripheral blood mononuclear cells and whole blood from 13 patients with
M. abscessus
pulmonary disease and 13 healthy controls to investigate their cytokine production after 24 h and 7 days.
Patients were predominantly women (54%) with a mean age of 59 years; 62% had nodular bronchiectatic disease. Many patients had predisposing pulmonary diseases, such as COPD (46%), and asthma (23%). Patients with COPD showed an impaired interleukin (IL)-6 response to
M. abscessus
and a reduced IL-17 response to
Candida
, together with a
M. abscessus
-specific enhanced IL-22 production. Patients without COPD showed higher levels of interleukin-1 receptor antagonist (IL-1Ra), an anti-inflammatory molecule. Within the non-COPD patients, those with bronchiectasis showed defective interferon (IFN)-γ production in response to
Candida albicans
.
In conclusion, susceptibility to
M. abscessus
is likely determined by a combination of immunological defects and predisposing pulmonary disease. The main defect in the innate immune response was a shift of the ratio of IL-1β to IL-1Ra, which decreased the bioactivity of this pathway in the adaptive immune response. In the adaptive immune response there was defective IL-17 and IFN-γ production. Patients with COPD and bronchiectasis showed different cytokine defects. It is therefore crucial to interpret the immunological results within the clinical background of the patients tested. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 2312-0541 2312-0541 |
DOI: | 10.1183/23120541.00590-2020 |