Cell interactions in the primary immune response in vitro: a requirement for specific cell clusters

Mouse spleen cells were found to associate in cell clusters during the primary immune response to sheep erythrocytes in vitro. About 10% of the cell clusters had the following unique properties; (a) they contained most, if not all, antibody-forming cells, (b) they contained only cells forming antibo...

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Bibliographic Details
Published inThe Journal of experimental medicine Vol. 129; no. 2; pp. 351 - 362
Main Author Mosier, D E
Format Journal Article
LanguageEnglish
Published United States The Rockefeller University Press 01.02.1969
Subjects
Animals
Antibody Formation
Antigen-Antibody Reactions
Carbon Isotopes
Cell Aggregation
Cell Membrane
Culture Techniques
Erythrocytes
Lymphocytes - immunology
Mice
Mitosis
Sheep
Spleen - immunology
Surface Properties
Thymidine
Time Factors
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Summary:Mouse spleen cells were found to associate in cell clusters during the primary immune response to sheep erythrocytes in vitro. About 10% of the cell clusters had the following unique properties; (a) they contained most, if not all, antibody-forming cells, (b) they contained only cells forming antibody to one antigen when cell cultures were immunized with two antigens, (c) the cells in clusters reaggregated specifically after dispersion, and (d) the specific reaggregation of clusters appeared to be blocked by antibody to the antigen. The integrity of cell clusters was required for the proliferation of antibody-forming cells, and prevention of clustering by mechanical means or by excess antibody blocked the immune response. Antibody and antigenic determinants on the surfaces of cells probably provide the basis for interaction. The unique microenvironment of cell clusters was essential for the primary immune response in vitro.
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ISSN:0022-1007
1540-9538
DOI:10.1084/jem.129.2.351