Expression of TIGIT in splenic and circulatory T cells from mice acutely infected with Toxoplasma gondii

• Published in: Parasite (Paris) Vol. 28; p. 13
• Main Authors: Wang, Shuai, Li, Haoran, Zhang, Fuqiang, Jiao, Yuankai, Xie, Qing, Zhang, Zhenchao, Li, Xiangrui
• Format: Journal Article
• Language: English
• Published: France EDP Sciences 2021
• Subjects: Animals; cd226; CD4 antigen; CD8 antigen; CD8-Positive T-Lymphocytes - immunology; Cell surface; Coloration; Flow cytometry; Immune response (cell-mediated); Immune system; Immunoreceptor tyrosine-based inhibition motif; Infections; Lesions; Leukocytes (mononuclear); Leukocytes, Mononuclear - immunology; Lymphocytes; Lymphocytes T; Mice; Peripheral blood mononuclear cells; Phenotypes; Protozoa; Real time; Receptors, Immunologic - genetics; Spleen; Spleen - cytology; t cells; tigit; Toxoplasma; Toxoplasma gondii; Toxoplasmosis - immunology; Tyrosine; CD226; Toxoplasma gondii; T cells; TIGIT
• ISSN: 1776-1042; 1252-607X; 1776-1042
• DOI: 10.1051/parasite/2021010

The surface protein TIGIT (T cell immunoglobulin and immunoreceptor tyrosine-based inhibitory motif (ITIM) domain) has been characterized as an important regulator of cell-mediated immune responses in various infections. However, TIGIT expression in immune cells of mice infected with Toxoplasma gondii has not been investigated. Here, we detected TIGIT expression and related phenotypes by flow cytometry and real-time PCR in splenic and circulatory T cells of mice infected with the T. gondii RH strain. We found that the expression of TIGIT on the surface of CD4 T cells and CD8 T cells from the spleen and peripheral blood mononuclear cells decreased in the early stage, but increased significantly in the late stage of acute T. gondii infection in mice. Importantly, TIGIT expression was positively correlated with lesions in the murine spleen. In addition, T. gondii-specific TIGIT T cells in the spleen were activated and transformed into TIGIT T cells. Hematoxylin and eosin staining of spleen sections and real-time PCR showed that the severity of splenic lesions was positively correlated with the T. gondii load. This study demonstrates that acute T. gondii infection can regulate the expression of TIGIT in T cells and affect immune cell function.