Antimicrobial Susceptibility and Characterization of Outer Membrane Proteins of Aeromonas hydrophila Isolated in China

• Published in: Journal of Integrative Agriculture Vol. 13; no. 4; pp. 911 - 917
• Main Author: GUO Peng, WANG Na, LIU Yong-jie , LU Cheng-ping (Institute of Geographic Sciences and Natural Resources Research Chinese Academy of Sciences Beijing 100101, P.R.China)
• Format: Journal Article
• Language: English
• Published: Science Press 01.04.2014; College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, P.R.China; Elsevier
• Subjects: Aeromonas hydrophila; Aeromonas hydrophila antimicrobial resistance outer membrane proteins; antimicrobial resistance; OMP型; outer membrane proteins; 分离株; 十二烷基硫酸钠; 嗜水气单胞菌; 外膜蛋白; 氟喹诺酮类药物; 磺胺间甲氧嘧啶; 表征; Aeromonas hydrophila; antimicrobial resistance; outer membrane proteins
• ISSN: 2095-3119; 2352-3425
• DOI: 10.1016/S2095-3119(13)60312-3

Aeromonas hydrophila isolates from clinical cases （n=43） were tested against 8 antimicrobial agents and typed by outer membrane protein （OMP） pattern by using sodium dodecyl sulfate gel electrophoresis. All isolates were resistant to ampicillin （MICs, ≥16 μg mL-1） and sulfamonomethoxine （MICs≥64 μg mLl）, but susceptible to norfloxacin （MICs,≤0.5 μg mL-1）. There was a high incidence of resistance to erythromycin （90.70%） and tylosin （93.02%）, while a low incidences of resistance to ciprofloxacin （2.33%）, enrofloxacin （2.33%） and florfenicol （4.65%）. Six different outer membrane protein patterns were found among 34 isolates by analyzing proteins in the range of 22 to 50 kDa, other than 9 isolates with their respective profiles. The strains with the similar OMP profiles had similar resistances. Compared with the other strains from the same OMP patterns, NB-1, A.Pun and MR-1 had lacked the proteins in the range of 30 to 45 kDa and their resistance to florfenicol substantially increased. It is speculated that the outer membrane protein changes might correlate with decreased susceptibility to florfenicol in the three strains. Some strains which showed completely identical OMP types had a little difference in their resistance to fluoroquinolones, indicating that there might be other factors that were involved in the antimicrobial resistance of A. hydrophila.