Plasmodium falciparum Gametocyte Density and Infectivity in Peripheral Blood and Skin Tissue of Naturally Infected Parasite Carriers in Burkina Faso

• Published in: The Journal of infectious diseases Vol. 223; no. 10; pp. 1822 - 1830
• Main Authors: Meibalan, Elamaran, Barry, Aissata, Gibbins, Matthew P, Awandu, Shehu, Meerstein-Kessel, Lisette, Achcar, Fiona, Bopp, Selina, Moxon, Christopher, Diarra, Amidou, Debe, Siaka, Ouédraogo, Nicolas, Barry-Some, Ines, Badoum, Emilie S, Fagnima, Traoré, Lanke, Kjerstin, Gonçalves, Bronner P, Bradley, John, Wirth, Dyann, Drakeley, Chris, Guelbeogo, Wamdaogo Moussa, Tiono, Alfred B, Marti, Matthias, Bousema, Teun
• Format: Journal Article
• Language: English
• Published: US Oxford University Press 28.05.2021
• Subjects: Animals; Anopheles - parasitology; Biopsy; Burkina Faso; Confocal microscopy; Culicidae; Disease transmission; Feeding; Gametocytes; Humans; Infectivity; Major and Brief Reports; Malaria; Malaria, Falciparum - epidemiology; Mosquitoes; Peripheral blood; Plasmodium falciparum; Skin; Burkina Faso; gametocyte; transmission; anopheles; elimination; sequestration; Plasmodium falciparum
• ISSN: 0022-1899; 1537-6613; 1537-6613
• DOI: 10.1093/infdis/jiz680

Abstract Background Plasmodium falciparum transmission depends on mature gametocytes that can be ingested by mosquitoes taking a blood meal on human skin. Although gametocyte skin sequestration has long been hypothesized as important contributor to efficient malaria transmission, this has never been formally tested. Methods In naturally infected gametocyte carriers from Burkina Faso, we assessed infectivity to mosquitoes by direct skin feeding and membrane feeding. We directly quantified male and female gametocytes and asexual parasites in finger-prick and venous blood samples, skin biopsy samples, and in of mosquitoes that fed on venous blood or directly on skin. Gametocytes were visualized in skin tissue with confocal microscopy. Results Although more mosquitoes became infected when feeding directly on skin then when feeding on venous blood (odds ratio, 2.01; 95% confidence interval, 1.21–3.33; P = .007), concentrations of gametocytes were not higher in the subdermal skin vasculature than in other blood compartments; only sparse gametocytes were observed in skin tissue. Discussion Our data strongly suggest that there is no significant skin sequestration of P. falciparum gametocytes. Gametocyte densities in peripheral blood are thus informative for predicting onward transmission potential to mosquitoes and can be used to target and monitor malaria elimination initiatives. Plasmodium falciparum gametocyte skin sequestration has long been hypothesized but never formally tested. We observed no evidence for higher gametocyte densities in skin tissue of naturally infected gametocyte carriers or blood meals of mosquitoes feeding on their skin.