Knockdown of miR-155 alleviates skin damage in rats with chronic spontaneous urticaria by modulating the JAK/STAT signaling pathway

• Published in: Allergy, asthma, and clinical immunology Vol. 20; no. 1; pp. 38 - 10
• Main Authors: An, Yue-Peng, Yuan, Rui, Wang, Shan-Shan, Yang, Su-Qing, Zhang, Qing
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 29.06.2024; BioMed Central; BMC
• Subjects: Autoimmunity; B cells; Chronic urticaria; Cytokines; Histamine; Immunoglobulin A; Immunoglobulin G; JAK/STAT signaling pathway; miR-155; Omalizumab; Proteins; Skin; T cells; Urticaria; China; Autoimmunity; JAK/STAT signaling pathway; Chronic urticaria; miR-155
• ISSN: 1710-1484; 1710-1492; 1710-1492
• DOI: 10.1186/s13223-024-00902-x

The aim of this study was to investigate the role and mechanisms of miR-155 in chronic spontaneous urticaria (CSU). The expression level of miR-155 in the skin tissues of patients with CSU and experimental rats were detected by RT-qPCR, followed by the measurement of the histamine release rate in the serum through the histamine release test. Besides, hematoxylin & eosin staining was used to observe the pathological changes of the skin tissues; Corresponding detection kits and flow cytometry to measure the changes of immunoglobulins, inflammatory cytokines and T cell subsets in the serum of rats in each group; and western blot to check the expression level of proteins related to JAK/STAT signaling pathway in the skin tissues. Knockdown of miR-155 reduced the number and duration of pruritus, alleviated the skin damage, and decreased the number of eosinophils in CSU rats. Moreover, knockdown of miR-155 elevated the serum levels of IgG and IgM, decreased the levels of IgA and inflammatory cytokines, and reduced the proportion of CD4 + and CD4 + CD25 + T cells, as well as the CD4+/CD8 + ratio in CSU rats. However, Tyr705 intervention could reverse the effects of knockdown of miR-155 on CSU model rats. Furthermore, we found that knockdown of miR-155 significantly reduced the protein expression of IRF-9, as well as the P-JAK2/JAK2 and P-STAT3/STAT3 ratios in the skin tissues of CSU rats. Knockdown of miR-155 can alleviate skin damage and inflammatory responses and relieve autoimmunity in CSU rats by inhibiting the JAK/STAT3 signaling pathway.