Application of monoclonal antibodies to quality control of foot-and-mouth disease vaccines

• Published in: Vaccine Vol. 12; no. 8; pp. 682 - 686
• Main Authors: Alonso, A., Darsie, G.C., Teixeira, A.C., Reis, J.L., Mesquita, J.A.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 1994; Elsevier
• Subjects: Animals; Antibodies, Monoclonal; Antibodies, Viral; Aphthovirus - immunology; Biological and medical sciences; Cattle; Cattle Diseases - prevention & control; ELISA; Enzyme-Linked Immunosorbent Assay - methods; Foot-and-mouth disease; Foot-and-Mouth Disease - prevention & control; Fundamental and applied biological sciences. Psychology; Microbiology; monoclonal antibodies; Neutralization Tests; Quality Control; vaccines; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Viral Vaccines - immunology; Viral Vaccines - standards; Virology; Foot-and-mouth disease; vaccines; monoclonal antibodies; ELISA; Virus; Performance evaluation; Immunoprotection; Aphthovirus; Vertebrata; Mammalia; Mouse; Picornaviridae; Rodentia; Quality control; Vaccine; Monoclonal antibody
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/0264-410X(94)90216-X

Panels of monoclonal antibodies (mAbs) produced against foot-and-mouth disease (FMD) virus types O, A and C were selected for cell culture neutralization titre (NT), mouse protection index (MPI), trypsin sensitivity (TS) and avidity to different epitopes. The selected sets were used to assay the antigen concentration and the fit between FMDV vaccine and challenge strains. It was observed that FMD vaccines protect more than 75% of vaccinated cattle when manufactured with antigens characterized by (1) a high degree of fit with the potency control virus, and (2) mean ELISA 50% titres ( T 50) > 28 for O, > 18 for A and > 75 for C types, respectively, using the corresponding mAb set.