Figures of Merit in the Quantification of Ibuprofen Enantiomers by Chiral HPLC

• Published in: Journal of chromatographic science Vol. 47; no. 9; pp. 777 - 780
• Main Authors: Valderrama, Patrícia, Romero, Adriano Lopes, Imamura, Paulo Mitsuo, Poppi, Ronei Jesus
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Niles, IL Oxford University Press 01.10.2009; Preston Publications
• Subjects: Analysis; Biological and medical sciences; Chromatography, High Pressure Liquid - methods; General pharmacology; Ibuprofen - chemistry; Medical sciences; Pharmacology. Drug treatments; Stereoisomerism; Validation; Prostaglandin-endoperoxide synthase; Chemical analysis; Separation; Enzyme; Enzyme inhibitor; HPLC chromatography; Purity control; Chiral stationary phase; Non steroidal antiinflammatory agent; Ultraviolet detector; Optical resolution; Enantiomer; Quality control; Cellulose derivatives; Ibuprofen; Arylpropionic acid derivatives; Optical purity; Oxidoreductases; Figure of merit; Quantitative analysis
• ISSN: 0021-9665; 1945-239X
• DOI: 10.1093/chromsci/47.9.777

The determination of ibuprofen (IBU) enantiomers by chiral high-performance liquid chromatographic is described. The methodology is based on chiral recognition of ibuprofen by a chiral column based on cellulose tris(4-methylbenzoate) coated on silica gel (Chiralcel OJ-H). The mobile phase is n-hexane-2-propanol-trifluoroacetic acid (98:2:0.1, v/v/v). The flow rate was 1.0 mL/min, and UV detection was 254 nm. The samples of ibuprofen were prepared in n-hexane in the concentration range 50–100% of (S)-IBU 1 × 10-3 mol/L. Calibration and validation method were performed with six and nine samples, respectively. Goodness-of-fit measures represented by correlation coefficient, y-intercept, and slope of the regression line were 0.9836, 21373, 2162, respectively. Average of the relative error of the proposed method was 3.0%, 0.9% (S)-IBU selectivity, and 2162% (S)-IBU-1 sensitivity. The minimum concentration difference between two samples that could be determined in the linear dynamic range was 0.4% (S)-IBU. Limits of detection and quantification were 8.1 and 27.0% (S)-IBU, respectively. These results indicate that the proposed method can be employed for determination of the enantiomeric composition of IBU.