Description of two novel alleles HLA-Cw0766 and Cw0767 identified in a Chinese Han individual by sequence-based typing

• Published in: Human immunology Vol. 71; no. 1; pp. 93 - 95
• Main Authors: Deng, Zhihui, Xu, Yunping, Wang, Daming, Jin, Shizheng
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 2010
• Subjects: Alleles; Allergy and Immunology; Asian Continental Ancestry Group - genetics; Base Sequence; Female; HLA-C Antigens - genetics; HLA-Cw0766; HLA-Cw0767; Humans; Male; Molecular Sequence Data; Novel allele; Sequence Alignment; Sequence-based typing; HLA-Cw0767; Sequence-based typing; Novel allele; HLA-Cw0766
• ISSN: 0198-8859; 1879-1166
• DOI: 10.1016/j.humimm.2009.10.008

Abstract We report two novel HLA-C alleles, Cw*0766, and Cw*0767 identified in a Chinese voluntary stem cell donor. This sample was initially found by direct PCR-SBT with an inconclusive result. To clarify this unusual SBT result, the PCR product of HLA-C gene covering from exon 1–4 was subjected to cloning and haplotype sequencing and family study of HLA inheritance consisting of six members were confirmed. Novel Cw*0766 allele differs from the closest allele Cw*07020101 by single nucleotide change at genomic DNA nt 1651 C>A (codon 206 CTG>ATG) in exon 4, which results in an amino acid change Leu206Met. Another novel allele Cw*0767 differs from the closest allele Cw*07020103 by single nucleotide change at genomic DNA nt 930 A>T (codon 161 GAG>GTG) in exon 3, which results in an amino acid change Glu161Val. Family study demonstrated that the novel Cw*0766 allele segregating on an A*2402 B*4001 Cw*0766 DRB1*1202 haplotype was inherited from the mother of the propositus, whereas the novel Cw*0767 allele on haplotype A*1101 B*0702 Cw*0767 DRB1*0101 was inherited from the father.