generation of GLP-grade human embryonic stem cell banks from four clinical-grade cell lines for preclinical research

• Published in: In vitro cellular & developmental biology. Animal Vol. 46; no. 3-4; pp. 210 - 216
• Main Authors: Sivarajah, Shirani, Raj, Grace Selva, Mathews, Adam Joshua Varughese, Sahib, Norliza Binte Esmail, Hwang, Wei Sek, Crook, Jeremy Micah
• Format: Journal Article
• Language: English
• Published: New York New York : Springer-Verlag 01.04.2010; Springer Science + Business Media; Springer-Verlag; Society for In Vitro Biology
• Subjects: Animal Genetics and Genomics; Biomarkers - metabolism; Biomedical and Life Sciences; Cell Biology; Cell Culture; Cell Culture Techniques - methods; Cell Differentiation - genetics; Cell Line; Cell lines; Developmental Biology; Embryo cells; Embryo Research; Embryonic stem cells; Embryonic Stem Cells - cytology; Ethics; Feeder cells; Fibroblasts; Flow Cytometry; Gene Expression Regulation, Developmental; Humans; Immunohistochemistry; Karyotyping; Life Sciences; Pluripotent stem cells; Quality assurance; Quality control; Reverse Transcriptase Polymerase Chain Reaction; Stem Cells; Straw; Teratoma; Tissue Banks - standards; Translation; Human embryonic stem cells; Banks; Clinical grade; Good laboratory practice; Research grade
• ISSN: 1071-2690; 1543-706X
• DOI: 10.1007/s11626-010-9287-1

The Singapore Stem Cell Bank has generated human embryonic stem cell banks from clinical-grade cell lines ESI-017, ESI-035, ESI-049, and ESI-053. All banks were prepared and characterized according to principles of Good Laboratory Practice for quality assurance. Importantly, each cell line has clearly documented and approved ethical provenance and meets recognized standards for performance and safety. The banks are intended to facilitate the translation of stem cell research to clinical medicine by enabling early phase research and development with high-quality, low-cost cells that are also available as clinical-grade stocks.