Effects of heat killed Lactobacillus plantarum (LP20) supplemental diets on growth performance, stress resistance and immune response of red sea bream, Pagrus major

• Published in: Aquaculture Vol. 442; pp. 29 - 36
• Main Authors: Dawood, Mahmoud A.O., Koshio, Shunsuke, Ishikawa, Manabu, Yokoyama, Saichiro
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.05.2015; Elsevier Sequoia S.A
• Subjects: Aquaculture; Blood parameters; Dietary supplements; Fish; Gram-positive bacteria; Growth performance; Heat killed bacteria; Immune response; Immunology; Lactobacillus plantarum; Marine; Pagrus major; Physical growth; Red sea bream; Stress response; Blood parameters; Red sea bream; Lactobacillus plantarum; Immune response; Growth performance; Heat killed bacteria
• ISSN: 0044-8486; 1873-5622
• DOI: 10.1016/j.aquaculture.2015.02.005

A feeding trial was carried out to determine effects of heat-killed Lactobacillus plantarum (HK-LP) for red sea bream (Pagrus major). Five dietary levels of a commercial product containing 20% HK-LP at 1, 10, 100, 1000, and 2000mgkg−1 diets were supplemented to the basal diet (control), respectively. Triplicate groups of fish (initial weight: 11g) were stocked in 100-L polycarbonate circular tanks at a density of 12 fish per tank under the flow-through system, and were fed the respective test diets for 56days. At the end of a feeding trial, the results showed that the fish fed the diet at 10, 100, 1000 and 2000mgkg−1 HK-LP significantly grew faster than control group. Similarly, significantly improved feed intake, feed efficiency ratio, protein retention, and apparent digestibility coefficients were also found at 1000mgkg−1 HK-LP group than those in HK-LP free group. Some parameters such as serum lysozyme activity, total serum protein, mucus secretion, and the tolerance against low salinity stress were improved in fish fed 1000mgkg−1 HK-LP compared to those in HK-LP free group. This study demonstrated that HK-LP enhanced non-specific immune defense system of red sea bream, providing them with higher resistance to the stress and better immune response. •No previous work has addressed the effect of probiotic for Pagrus major.•Fish performances were improved by heat-killed Lactobacillus plantarum (HK-LP).•Enhancement of Pagrus major immune responses by HK-LP•Enhancement of antioxidant status of Pagrus major•The optimum supplement of dietary HK-LP for Pagrus major is 0.1%.