Ultrastructural localization of antibody in differentiating plasma cells

• Published in: The Journal of experimental medicine Vol. 127; no. 1; pp. 109 - 118
• Main Authors: Leduc, E H, Avrameas, S, Bouteille, M
• Format: Journal Article
• Language: English
• Published: United States The Rockefeller University Press 01.01.1968
• Subjects: Animals; Antibodies - analysis; Antigens; Cytoplasm - immunology; Cytoplasmic Granules - immunology; Golgi Apparatus - immunology; Histocytochemistry; Microscopy, Electron; Peroxidases; Plasma Cells - immunology; Rabbits; Spleen - immunology
• ISSN: 0022-1007; 1540-9538
• DOI: 10.1084/jem.127.1.109

Antibody was localized by electron microscopy within differentiating and mature plasma cells of the spleens of hyperimmunized rabbits. Horseradish peroxidase was used as antigen. Intracellular antibody to peroxidase was revealed in glutaraldehyde-fixed tissue by coupling it with its antigen and then revealing the sites of peroxidase activity cytochemically. Antibody first appears in the perinuclear space of hemocytoblasts where it persists through differentiation into immature plasma cells, but it disappears from this site in mature plasma cells. Concomitant with the development of the ergastoplasm, antibody accumulates in many but not all of its cisternae. Antibody is present in the lamellar portion of the Golgi apparatus in all phases of plasmacytic differentiation. Mature plasma cells exhibit two types of antibody distribution, a concentration into large spherical intracisternal granules or an overflowing into all parts of the cytoplasm.