A Sensitive Gas Chromatographic Assay for Cystathionase and Cystathionine : Application of This Assay to Biological Samples and Kinetic Studies. Gas Chromatographic Assay for Cystathionase and Cystathionine

α-Ketobutyric acid is one of the reaction products formed from cystathionine or homoserine by cystathionase. α-Ketobutyric acid was reacted with pentafluorophenylhydrazine, and the hydrazone was extracted with ethyl ether, reacted with diazomethane, and subjected to gas-liquid chromatography with an...

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Published inChemical & pharmaceutical bulletin Vol. 30; no. 6; pp. 2099 - 2104
Main Authors OHMORI, SHINJI, MIZUNO, SACHIKO, IKEDA, MIKIKO, YAO, KENZABURO
Format Journal Article
LanguageEnglish
Published Japan The Pharmaceutical Society of Japan 01.01.1982
Subjects
Animals
Chromatography, Gas
Cystathionine - analysis
Cystathionine gamma-Lyase - metabolism
ECD-gas chromatography
Kinetics
Lyases - metabolism
Rats
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Summary:α-Ketobutyric acid is one of the reaction products formed from cystathionine or homoserine by cystathionase. α-Ketobutyric acid was reacted with pentafluorophenylhydrazine, and the hydrazone was extracted with ethyl ether, reacted with diazomethane, and subjected to gas-liquid chromatography with an electron capture detector. This method provides a specific and sensitive assay for cystathionase and cystathionine in biological samples.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0009-2363
1347-5223
DOI:10.1248/cpb.30.2099