MicroRNA-210-3p Promotes Chondrogenic Differentiation and Inhibits Adipogenic Differentiation Correlated with HIF-3α Signalling in Bone Marrow Mesenchymal Stem Cells

• Published in: BioMed research international Vol. 2021; pp. 6699910 - 8
• Main Authors: Yang, Meng, Yan, Xin, Yuan, Fu-Zhen, Ye, Jing, Du, Ming-Ze, Mao, Zi-Mu, Xu, Bing-Bing, Chen, You-Rong, Song, Yi-Fan, Fan, Bao-Shi, Yu, Jia-Kuo
• Format: Journal Article
• Language: English
• Published: United States Hindawi 2021; Hindawi Limited
• Subjects: Adipogenesis - genetics; Animals; Base Sequence; Binding Sites; Biomedical materials; Bone marrow; Cartilage; Cartilage (articular); Cartilage diseases; Cell differentiation; Chondrogenesis - genetics; Differentiation; Efficiency; Extracellular matrix; Flow cytometry; Gene expression; Genes; Male; Mesenchymal stem cells; Mesenchymal Stem Cells - cytology; Mesenchymal Stem Cells - metabolism; MicroRNAs; MicroRNAs - genetics; MicroRNAs - metabolism; miRNA; Models, Biological; mRNA; Osteoarthritis; Protein expression; Proteins; Rats; Rats, Sprague-Dawley; Regeneration; Ribonucleic acid; RNA; RNA, Messenger - genetics; RNA, Messenger - metabolism; Signal Transduction; Signaling; Sox9 protein; Statistical analysis; Stem cell transplantation; Stem cells; Therapeutic targets; Transcription Factors - genetics; Transcription Factors - metabolism; United Kingdom > UK; United States > US; China
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2021/6699910

Cartilage injury of the knee joint is very common. Due to the limited self-healing ability of articular cartilage, osteoarthritis is very likely to occur if left untreated. Bone marrow mesenchymal stem cells (BMMSCs) are widely used in the study of cartilage injury due to their low immunity and good amplification ability, but they still have disadvantages, such as heterogeneous undifferentiated cells. MicroRNAs can regulate the chondrogenic differentiation ability of MSCs by inhibiting or promoting mRNA translation and degradation. In this research, we primarily investigated the effect of microRNA-210-3p (miR-210-3p) on chondrogenic and adipogenic differentiation of BMMSCs in vitro. Our results demonstrate that miR-210-3p promoted chondrogenic differentiation and inhibited adipogenic differentiation of rat BMMSCs, which was related to the HIF-3α signalling pathway. Additionally, miR-210-3p promotes mRNA and protein levels of the chondrogenic expression genes COLII and SOX9 and inhibits mRNA and protein levels of the adipogenic expression genes PPARγ and LPL. Thus, miR-210-3p combined with BMMSCs is a candidate for future clinical applications in cartilage regeneration and could represent a promising new therapeutic target for OA.