Spray dried human and chimpanzee adenoviral-vectored vaccines are thermally stable and immunogenic in vivo

• Published in: Vaccine Vol. 35; no. 22; pp. 2916 - 2924
• Main Authors: Afkhami, Sam, LeClair, Daniel A, Haddadi, Siamak, Lai, Rocky, Toniolo, Steven P, Ertl, Hildegund C, Cranston, Emily D, Thompson, Michael R, Xing, Zhou
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 19.05.2017; Elsevier Limited
• Subjects: Adenoviral-vectored; Adenoviridae - genetics; Adenoviruses, Simian; Allergy and Immunology; Ambient temperature; Animal model; Animals; Antigens; Chemical properties; Cold storage; Cryopreservation; Desiccation; Dextran; Drug delivery systems; Drug Storage; Drying; Genomes; Humans; Humidity; Immunogenicity; Immunogenicity, Vaccine; Infections; Infectivity; Mannitol; Mathematical analysis; Matrix methods; Morphology; Pan troglodytes; Particle size; Powders; Process controls; Proteins; Spray drying; Supply chains; Temperature; Thermal stability; Thermostability; Trehalose; Tuberculosis; Tuberculosis Vaccines - administration & dosage; Tuberculosis Vaccines - chemistry; Tuberculosis Vaccines - immunology; Vaccine; Vaccine Potency; Vaccines; Vaccines, Synthetic - chemistry; Vaccines, Synthetic - immunology; Virology; Animal model; AdCh68; AdHu5; volume median diameter; DSC; relative standard deviation; T g; Ag85A; chimpanzee serotype 68 adenoviral vector; PBS; scanning electron microscopy; CO 2; carbon dioxide; UV; RSD; plaque forming unit; Spray drying; Vaccine; Mycobacterium tuberculosis antigen 85A; glass transition temperature; TB; Thermostability; human serotype 5 adenoviral vector; Adenoviral-vectored; Tuberculosis; Mycobacterium tuberculosis; differential scanning calorimetry; phosphate buffered saline; ultraviolet; d(0.5); SEM; pfu; M. tb; CO2; Tg
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2017.04.026

Highlights • Human and chimpanzee adenoviral-vectored vaccines spray dried in a sugar matrix. • Spray dried vaccine powders demonstrate thermostability and in vivo efficacy. • Spray dried vaccine powders when stored, cold chain-free, are stable up to 90 days.