Transfection of Difficult-to-Transfect Rat Primary Cortical Neurons with Magnetic Nanoparticles

The efficient cell transfection method is vital for various biomedical applications, such as the CRISPR-Cas9 technique. Current cell transfection methods, including lipofectamine, calcium phosphate co-precipitation, nucleofection, and viral infection are not equally efficient for various cells and h...

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Published inJournal of biomedical nanotechnology Vol. 14; no. 9; p. 1654
Main Authors Jin, Wuyang, Lin, Donghai, Nguyen, Anh H, Abdelrasoul, Gaser N, Chen, Jian, Mar, Arthur, Qian, Feng, Fang, Qiang, Kovalchuk, Igor, Wang, Yutian, Chen, Jie
Format Journal Article
LanguageEnglish
Published United States 01.09.2018
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Summary:The efficient cell transfection method is vital for various biomedical applications, such as the CRISPR-Cas9 technique. Current cell transfection methods, including lipofectamine, calcium phosphate co-precipitation, nucleofection, and viral infection are not equally efficient for various cells and have their disadvantages. In this study, a magnetic nanoparticle (MNP)-based method was introduced for delivering both FITC dye and a functional EGFP gene into easy-to-transfect HEK cells and difficult-to-transfect rat primary cortical neurons. The transfection efficacy could be controlled in both time-dependent and magnetic strength-dependent manner. This cell transfection method could have substantial potential for targeted drug delivery.
ISSN:1550-7033
DOI:10.1166/jbn.2018.2604