Identification and expression of cellobiohydrolase (CBHI) gene from an endophytic fungus, Fusicoccum sp. (BCC4124) in Pichia pastoris

• Published in: Protein expression and purification Vol. 58; no. 1; pp. 148 - 153
• Main Authors: Kanokratana, Pattanop, Chantasingh, Duriya, Champreda, Verawat, Tanapongpipat, Sutipa, Pootanakit, Kusol, Eurwilaichitr, Lily
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.03.2008
• Subjects: Ascomycota; Ascomycota - enzymology; Cellobiohydrolase; Cellulose 1,4-beta-Cellobiosidase - genetics; Cellulose 1,4-beta-Cellobiosidase - isolation & purification; Cellulose 1,4-beta-Cellobiosidase - metabolism; Cellulose binding domain; Cloning, Molecular; Endophytic fungus; Fusicoccum; Gene Expression; Genes, Fungal; Pichia - genetics; Pichia - metabolism; Pichia pastoris; Recombinant Proteins - isolation & purification; Recombinant Proteins - metabolism; Fusicoccum; Pichia pastoris; Endophytic fungus; Cellulose binding domain; Cellobiohydrolase

A gene encoding a cellobiohydrolase (CBHI) was isolated from Fusicoccum sp. (BCC4124), an endophytic fungus belongs in phylum Ascomycota, using 5′ and 3′ rapid amplification of cDNA end (RACE) technique. This CBHI gene contains 1395 nucleotides and encodes a 465-amino acid protein with a molecular w...