Manipulating Living Cells to Construct Stable 3D Cellular Assembly Without Artificial Scaffold

Regenerative medicine and tissue engineering offer several advantages for the treatment of intractable diseases, and several studies have demonstrated the importance of 3-dimensional (3D) cellular assemblies in these fields. Artificial scaffolds have often been used to construct 3D cellular assembli...

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Bibliographic Details
Published inJournal of visualized experiments no. 140
Main Authors Yamazaki, Takehiro, Taniguchi, Hiroaki, Tsuji, Shoto, Sato, Shiho, Kenmotsu, Takahiro, Yoshikawa, Kenichi, Sadakane, Koichiro
Format Journal Article
LanguageEnglish
Published United States MyJove Corporation 26.10.2018
Subjects
Biochemistry
Humans
Polymers
Regenerative Medicine
Tissue Engineering - methods
Tissue Scaffolds
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Summary:Regenerative medicine and tissue engineering offer several advantages for the treatment of intractable diseases, and several studies have demonstrated the importance of 3-dimensional (3D) cellular assemblies in these fields. Artificial scaffolds have often been used to construct 3D cellular assemblies. However, the scaffolds used to construct cellular assemblies are sometimes toxic and may change the properties of the cells. Thus, it would be beneficial to establish a non-toxic method for facilitating cell-cell contact. In this paper, we introduce a novel method for constructing stable cellular assemblies by using optical tweezers with dextran. One of the advantages of this method is that it establishes stable cell-to-cell contact within a few minutes. This new method allows the construction of 3D cellular assemblies in a natural hydrophilic polymer and is expected to be useful for constructing next-generation 3D single-cell assemblies in the fields of regenerative medicine and tissue engineering.
Bibliography:Correspondence to: Koichiro Sadakane at ksadakan@mail.doshisha.ac.jp
ISSN:1940-087X
1940-087X
DOI:10.3791/57815