Hyperbaric oxygen preconditioning promotes angiogenesis in rat liver after partial hepatectomy
Hyperbaric oxygen preconditioning (HBO-PC) increases the level of HIF-1α (hypoxia inducible factor-1α) and its target gene VEGF (vascular endothelial growth factor) which is involved in angiogenesis. Liver regeneration is an angiogenesis-dependent process. We hypothesized that HIF-1α and VEGF mediat...
Saved in:
Published in | Life sciences (1973) Vol. 83; no. 7; pp. 236 - 241 |
---|---|
Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
Elsevier Inc
15.08.2008
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Hyperbaric oxygen preconditioning (HBO-PC) increases the level of HIF-1α (hypoxia inducible factor-1α) and its target gene VEGF (vascular endothelial growth factor) which is involved in angiogenesis. Liver regeneration is an angiogenesis-dependent process. We hypothesized that HIF-1α and VEGF mediated the angiogenesis effect of HBO-PC on regenerating rat liver. Male Sprague Dawley rats received HBO-PC followed by 70% partial hepatectomy. Proliferation of hepatocytes and endothelial cells was evaluated by BrdU (bromodeoxyuridine) staining. Microvascular density was assessed by immunohistochemistry. mRNA expression of HIF-1α was assessed by quantitative RT-PCR and protein levels of HIF-1α and VEGF were assessed by western blot. HIF-1α DNA-binding activity was determined with an ELISA-based kit. HBO-PC increased the proliferation index of endothelial cells and microvascular density at 48 h after partial hepatectomy. The protein level and DNA-binding activity of HIF-1α and the protein level of VEGF were increased by HBO-PC before and after partial hepatectomy. Partial hepatectomy alone also increased proliferation index and the expressions of HIF-1α and VEGF. Our results indicated that the angiogenesis effect of HBO-PC on liver after partial hepatectomy could be achieved by increased HIF-1α activity and VEGF expression. However, the angiogenic effect of HBO-PC is moderate and HBO-PC failed to produce additional effect on the enhancement of HIF-1α and VEGF induced by partial hepatectomy alone. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0024-3205 1879-0631 |
DOI: | 10.1016/j.lfs.2008.06.011 |