Immunological and Enzymological Localization of Carbonyl Reductase in Ovary and Liver of Various Species

• Published in: Journal of biochemistry (Tokyo) Vol. 107; no. 2; pp. 209 - 212
• Main Authors: Iwata, Nobuhisa, Inazu, Norihisa, Satoh, Tetsuo
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.02.1990
• Subjects: Alcohol Dehydrogenase - immunology; Alcohol Dehydrogenase - metabolism; Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Blotting, Western; Cattle; Cricetinae; Dogs; Enzymes and enzyme inhibitors; Female; Fundamental and applied biological sciences. Psychology; Guinea Pigs; Haplorhini; liver; Liver - enzymology; Mice; ovary; Ovary - enzymology; Oxidoreductases; Pregnancy; Rabbits; Rats; Species Specificity; Substrate Specificity; Swine; Pregnancy; Ovary; Vertebrata; Mammalia; Enzymatic activity; Enzyme; Liver; Carbonyl reductase(NADPH); Immunoblotting assay; Localization; Comparative study
• ISSN: 0021-924X; 1756-2651
• DOI: 10.1093/oxfordjournals.jbchem.a123027

The tissue distribution of carbonyl reductase in ovary and liver of various animal species was investigated by measuring the reduction of 13,14-dihydro-15-keto-prostaglandin F2α9 a specific substrate for rat ovarian carbonyl reductases, and by means of Western blotting analysis using anti-rat ovarian carbonyl reductase antibody. The highest ovarian carbonyl reductase activity towards 13,14-dihydro-15-keto-PGF2α was found in rat among ten animal species tested, followed by hamster and monkey. The immunoreactive protein was detected in hamster and monkey ovaries. Although carbonyl reductase activity towards 13,14-dihydro-15-keto-PGF2α was not detectable in non-pregnant rabbit ovary, pregnant rabbit ovary showed not only moderate activity but also immunoreactivity with anti-rat ovarian carbonyl reductase antibody. On the other hand, carbonyl reductase activity towards 13,14-dihydro-15-keto-PGF2α was detected in hepatic tissue of all the species tested, except for rat and left-eye flounder. Immunoreactive proteins were present in hepatic tissue of various species that exhibited measurable carbonyl reductase activity towards 13,14-dihydro-15-keto-PGF2α