Synthesis, characterization and biological studies of rhenium, technetium-99m and rhenium-188 pentapeptides

• Published in: Nuclear medicine and biology Vol. 68-69; pp. 1 - 13
• Main Authors: Sanders, Vanessa A., Iskhakov, David, Abdel-Atti, Dalya, Devany, Matthew, Neary, Michelle C., Czerwinski, Ken R., Francesconi, Lynn C.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.01.2019; Elsevier BV
• Subjects: 188Re; 99mTc; Amino Acid Sequence; Animals; Arginine; Biological properties; Cadmium; Chelation; Chemical compounds; Chemistry Techniques, Synthetic; Coordination compounds; Crystallography; Decomposition; Female; High-performance liquid chromatography; Identification methods; In vivo methods and tests; KYCAR; Ligands; Liquid chromatography; Metal complexes; Metal peptide complex; Mice; Mice, Nude; NMR; Nuclear magnetic resonance; Oligopeptides - chemical synthesis; Oligopeptides - chemistry; Oligopeptides - pharmacokinetics; Pentapeptides; Pharmacology; Radiochemistry; Radioisotopes - chemistry; Radiometals; Rhenium; Rhenium - chemistry; Single photon emission computed tomography; SPECT; Stability analysis; Stomach; Synthesis; Technetium; Technetium - chemistry; Technetium isotopes; Thyroid; Tissue Distribution; Tomography, Emission-Computed, Single-Photon; Tracers; 188Re; 99mTc; Radiometals; KYCAR; Metal peptide complex; SPECT; (99m)Tc; Re
• ISSN: 0969-8051; 1872-9614
• DOI: 10.1016/j.nucmedbio.2018.11.001

A pentapeptide macrocyclic ligand, KYCAR (lysyl-tyrosyl-cystyl-alanyl-arginine), has been designed as a potential chelating ligand for SPECT imaging and therapeutic in vivo agents. This study shows the synthesis and characterization of KYCAR complexes containing nonradioactive rhenium, 99mTc, or 188Re. The metal complexes were also biologically evaluated to determine in vivo distribution in healthy mice. The overall goals of this project were (1) to synthesize the Tc/Re pentapeptide complexes, (2) to identify spectroscopic methods for characterization of syn versus anti rhenium peptide complexes, (3) to analyze the ex vivo stability, and (4) to assess the biological properties of the [99mTc]TcO-KYCAR and [188Re]ReO-KYCAR complexes in vivo. Details on these efforts are provided below. NatRe/99mTc/188ReO-KYCAR complexes were synthesized, and macroscopic species were characterized via HPLC, IR, NMR, and CD. These characterization data were compared to the crystallographic data of ReO-KYC to assist in the assignment of diastereomers and to aid in the determination of the structure of the complex. The radiometal complexes were synthesized with high purity (>95%). HPLC, IR, NMR and CD data on the macroscopic natReO-KYCAR complexes confirm the successful complexation as well as the presence of two diastereomers in syn and anticonformations. Tracer level complexes show favorable stabilities ex vivo for 2+ h. Macroscopic metal complexes form diastereomers with the KYCAR ligand; however, this phenomenon is not readily observed on the tracer level due to the rapid interconversion. It was determined through pKa measurements that the macroscopic natReO-KYCAR complex is 0 at physiological pH. The [99mTc]TcO-KYCAR is stable in vitro while the [188Re]ReO-KYCAR shows 50% decomposition in PBS and serum. Biologically, the tracer level complexes clear through the hepatobiliary pathway. Some decomposition of both tracers is evident by uptake in the thyroid and stomach.