Heterogeneity of Calcium Responses to Secretagogues in Corticotrophs From Male Rats

• Published in: Endocrinology (Philadelphia) Vol. 158; no. 6; pp. 1849 - 1858
• Main Authors: Romanò, Nicola, McClafferty, Heather, Walker, Jamie J., Le Tissier, Paul, Shipston, Michael J.
• Format: Journal Article
• Language: English
• Published: Washington, DC Endocrine Society 01.06.2017; Oxford University Press
• Subjects: Adrenocorticotropic Hormone - metabolism; Animals; Arginine; Arginine Vasopressin - metabolism; Arginine Vasopressin - pharmacology; Argipressin; Calcium; Calcium (intracellular); Calcium - metabolism; Calcium Signaling - drug effects; Calcium Signaling - genetics; Cells, Cultured; Coding; Corticosterone; Corticosterone - pharmacology; Corticotrophs - drug effects; Corticotrophs - metabolism; Corticotropin-releasing hormone; Corticotropin-Releasing Hormone - metabolism; Corticotropin-Releasing Hormone - pharmacology; Endocrinology; Genetic code; Heterogeneity; Hypothalamus; Hypothalamus - drug effects; Hypothalamus - metabolism; Male; Peptides; Physiology; Pituitary (anterior); Pituitary Hormones, Anterior - metabolism; Rats; Rats, Sprague-Dawley; Rats, Transgenic; Rodents; Sensitivity; Stimuli; Vasopressin
• ISSN: 0013-7227; 1945-7170
• DOI: 10.1210/en.2017-00107

Abstract Heterogeneity in homotypic cellular responses is an important feature of many biological systems, and it has been shown to be prominent in most anterior pituitary hormonal cell types. In this study, we analyze heterogeneity in the responses to hypothalamic secretagogues in the corticotroph cell population of adult male rats. Using the genetically encoded calcium indicator GCaMP6s, we determined the intracellular calcium responses of these cells to corticotropin-releasing hormone and arginine-vasopressin. Our experiments revealed marked population heterogeneity in the response to these peptides, in terms of amplitude and dynamics of the responses, as well as the sensitivity to different concentrations and duration of stimuli. However, repeated stimuli to the same cell produced remarkably consistent responses, indicating that these are deterministic on a cell-by-cell level. We also describe similar heterogeneity in the sensitivity of cells to inhibition by corticosterone. In summary, our results highlight a large degree of heterogeneity in the cellular mechanisms that govern corticotroph responses to their physiological stimuli; this could provide a mechanism to extend the dynamic range of the responses at the population level to allow adaptation to different physiological challenges. We show prominent heterogeneity in several aspects of the calcium responses to hypothalamic secretagogues in corticotrophs of adult male rats.