Separation, Purification, and Characterization of Analogues Components of a Commercial Sample of New Fuchsin

• Published in: Journal of chromatographic science Vol. 48; no. 8; pp. 618 - 623
• Main Authors: Montes de Oca, María N., Aiassa, Ivana M., Urrutia, María N., Argüello, Gerardo A., Ortiz, Cristina S.
• Format: Journal Article
• Language: English
• Published: Niles, IL Oxford University Press 01.09.2010; Preston Publications
• Subjects: Analysis; Analytical chemistry; Biological and medical sciences; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, High Pressure Liquid - methods; Chromatography, Reverse-Phase; Chromatography, Thin Layer; Exact sciences and technology; General pharmacology; Mass Spectrometry; Medical sciences; Nuclear Magnetic Resonance, Biomolecular; Other chromatographic methods; Pharmacology. Drug treatments; Rosaniline Dyes - chemistry; Rosaniline Dyes - isolation & purification; Spectrophotometry; Solvent effect; Tandem mass spectrometry; Chemical analysis; Purification; Preparative chromatography; Chemical stability; HPLC chromatography; Triarylmethane dye; Purity control; NMR spectrometry; Basic dye; Electrospray; Characterization; Hydrogen 1; Thin layer chromatography; Quality control; Commercial form; Spectrophotometry; Column chromatography; Mass spectrometry; Carbon 13; Ultraviolet visible spectrometry
• ISSN: 0021-9665; 1945-239X
• DOI: 10.1093/chromsci/48.8.618

New Fuchsin (NF), also known as Magenta III, has potential applications in photodynamic therapy. The commercial product labeled NF contains two other dye components in different proportions, Magenta II and Magenta I (Rosaniline). The proportions of NF, Magenta II, and Magenta I determined by reversed-phase high-performance liquid chromatography (RP-HPLC) in the commercial sample used were 71.6 ± 0.4%, 25.2 ± 0.2%, and 2.8 ± 0.1% (n = 7), respectively. The isolation, purification, and characterization of commercial NF dye components were carried out applying different techniques, such as preparative column liquid chromatography (PCLC), thin layer chromatography (TLC), RP-HPLC, absorption spectrophotometry, nuclear magnetic resonance spectroscopy (NMR), electrospray ionization mass spectrometry (ESI-MS), and tandem electrospray ionization mass spectrometry (ESI-MS-MS). After separation and isolation, the degree of purity obtained for NF compound was higher than 95% and 92% for Magenta II and Magenta I compounds, respectively. Therefore, it is essential to ensure a high degree of purity of these dyes as raw material to obtain new drugs intended for therapeutic treatments.