DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress

• Published in: BMC genomics Vol. 25; no. 1; p. 330
• Main Authors: Wang, Shuo, Zhao, XueLei, Li, Chang, Dong, Jing, Ma, JiaCheng, Long, YueHong, Xing, ZhaoBin
• Format: Journal Article
• Language: English
• Published: England BioMed Central 02.04.2024; BMC
• Subjects: Accumulation; Adaptability; Demethylation; Deoxyribonucleic acid; DNA; DNA biosynthesis; DNA methylation; Drought; Drought stress; Eleutherococcus senticosus; Gene expression; Genomes; Herbal medicine; Metabolism; Metabolites; Plant growth; Plants; Saponin; Saponins; Secondary metabolite; Secondary metabolites; Survival; Synthesis; Transcription; Transcription factor; Transcription factors; Water deprivation; Water shortages; United States > US; China; DNA methylation; Drought stress; Secondary metabolite; Transcription factor; Eleutherococcus senticosus; Saponin
• ISSN: 1471-2164; 1471-2164
• DOI: 10.1186/s12864-024-10237-x

Plant growth and development can be significantly impacted by drought stress. Plants will adjust the synthesis and accumulation of secondary metabolites to improve survival in times of water constraint. Simultaneously, drought stress can lead to modifications in the DNA methylation status of plants, and these modifications can directly impact gene expression and product synthesis by changing the DNA methylation status of functional genes involved in secondary metabolite synthesis. However, further research is needed to fully understand the extent to which DNA methylation modifies the content of secondary metabolites to mediate plants' responses to drought stress, as well as the underlying mechanisms involved. Our study found that in Eleutherococcus senticosus (E. senticosus), moderate water deprivation significantly decreased DNA methylation levels throughout the genome and at the promoters of EsFPS, EsSS, and EsSE. Transcription factors like EsMYB-r1, previously inhibited by DNA methylation, can re-bind to the EsFPS promotor region following DNA demethylation. This process promotes gene expression and, ultimately, saponin synthesis and accumulation. The increased saponin levels in E. senticosus acted as antioxidants, enhancing the plant's adaptability to drought stress.