Unveiling Ga(III) phthalocyanine—a different photosensitizer in neuroblastoma cellular model

• Published in: Journal of cellular and molecular medicine Vol. 23; no. 2; pp. 1086 - 1094
• Main Authors: Constantin, Carolina, Lupu, Andreea‐Roxana, Fertig, Tudor Emanuel, Gherghiceanu, Mihaela, Pop, Sevinci, Ion, Rodica‐Mariana, Neagu, Monica
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.02.2019; John Wiley and Sons Inc
• Subjects: Cell Line, Tumor; Cell Proliferation - radiation effects; Cell Survival - radiation effects; Confocal microscopy; Gallium Radioisotopes - pharmacology; gallium(III); Humans; Indoles - pharmacology; Microscopy; Neuroblastoma; Neuroblastoma - radiotherapy; Neuroblastoma cells; Original; Photochemotherapy - methods; Photodynamic therapy; photosensitizer; Photosensitizing Agents - pharmacology; proliferation; Toxicity; Toxicology; tumour; viability; Germany; toxicity; proliferation; photodynamic therapy; tumour; gallium(III); neuroblastoma; photosensitizer; viability
• ISSN: 1582-1838; 1582-4934
• DOI: 10.1111/jcmm.14009

Phthalocyanines (Pc) and their metallated derivatives are strongly considered for photodynamic therapy (PDT) possessing unique properties as possible new photosensitizers (PS). We have used toxicological assessments, real‐time monitoring of cellular impedance, and imagistic measurements for assessing the in vitro dark toxicity and PDT efficacy of Ga(III)‐Pc in SHSy5Y neuroblastoma cells. We have established the non‐toxic concentration range of Ga(III)‐Pc, a compound which shows a high intracellular accumulation, with perinuclear distribution in confocal microscopy. By choosing Ga(III)Pc non‐toxic dose, we performed in vitro experimental PDT hampering cellular proliferation. Our proposed Ga(III)‐Pc could complete a future PS panel for neuroblastoma alternate therapy.