Recent advances in hydrophilic interaction liquid chromatography (HILIC) for structural glycomics

• Published in: Electrophoresis Vol. 32; no. 24; pp. 3456 - 3466
• Main Authors: Zauner, Gerhild, Deelder, André M., Wuhrer, Manfred
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01.12.2011; WILEY‐VCH Verlag
• Subjects: Chromatography, Liquid; Electrophoresis, Capillary; Glycans; Glycomics; Glycopeptides; Glycopeptides - chemistry; Glycopeptides - metabolism; Glycoproteomics; HILIC; Hydrophobic and Hydrophilic Interactions; Polysaccharides - chemistry; Polysaccharides - metabolism; Solid Phase Extraction
• ISSN: 0173-0835; 1522-2683
• DOI: 10.1002/elps.201100247

This review presents recent progress in employing hydrophilic interaction liquid chromatography (HILIC) for glycan and glycopeptides analysis. After an introduction of this technique, the following themes are addressed: (i) implementation of HILIC in large‐scale studies for analyzing the human plasma N‐glycome; (ii) the use of HILIC UPLC (ultrahigh pressure liquid chromatography) for fast high‐resolution runs and its successful application with online MS for glycan and glycopeptide analysis; (iii) high‐throughput profiling using HILIC solid‐phase extraction in combination with MS detection; (iv) HILIC sample preparation for CE and CGE; (v) the latest glycoproteomic approaches implementing HILIC separation; (vi) future perspectives of HILIC including its use in large‐scale glycoproteomics studies such as the analysis of entire glycoproteomes at the glycopeptide level.