Prognostic factors in bladder carcinoma: histologic parameters and expression of a cell cycle-related nuclear antigen (Ki-67)

• Published in: The Journal of pathology Vol. 166; no. 1; p. 37
• Main Authors: Mulder, A H, Van Hootegem, J C, Sylvester, R, ten Kate, F J, Kurth, K H, Ooms, E C, Van der Kwast, T H
• Format: Journal Article
• Language: English
• Published: England 01.01.1992
• Subjects: Antigens, Neoplasm - analysis; Antigens, Nuclear; Carcinoma, Transitional Cell - immunology; Carcinoma, Transitional Cell - pathology; Cell Division - physiology; Humans; Immunoenzyme Techniques; Mitosis - physiology; Nuclear Proteins - analysis; Prognosis; Urinary Bladder Neoplasms - immunology; Urinary Bladder Neoplasms - pathology
• ISSN: 0022-3417
• DOI: 10.1002/path.1711660107

A total of 316 biopsies from patients with bladder carcinoma who entered a trial of the genito-urinary group of the European Organization for Research and Treatment of Cancer (EORTC) were reviewed. The histological data were subsequently correlated with the clinical course. A strong correlation between the number of mitoses and the time of first recurrence, muscle invasion, and death was noted. Next the expression of Ki-67 in frozen sections from 49 transitional cell carcinomas (TCCs) and the DNA content of the tumour cells were determined. The frequency of Ki-67-positive tumour cells increased with tumour grade and stage. Grade II TCCs and superficially infiltrating TCCs showed a wide range of Ki-67 scores. There was a significant difference in Ki-67 score between non-infiltrating (Ta) and superficially infiltrating (T1) grade II TCCs. All DNA-aneuploid carcinomas but also 15 out of 36 DNA-diploid tumours contained more than 10 per cent Ki-67-positive cells. Only some of these tumours were DNA-aneuploid or -tetraploid. The results indicate that the number of Ki-67-positive cells in grade II tumours may be a useful aid in separating grade II TCCs with a favourable prognosis from those with a poor clinical outcome.