Polysaccharides from Dicliptera chinensis ameliorate liver disturbance by regulating TLR‐4/NF‐κB and AMPK/Nrf2 signalling pathways

• Published in: Journal of cellular and molecular medicine Vol. 24; no. 11; pp. 6397 - 6409
• Main Authors: Zhang, Kefeng, Xu, Qiongmei, Gao, Ya, Cao, Houkang, Lian, Yuanyu, Li, Zimeng, Xu, Jie, Zhong, Mingli, Li, Jiani, Wei, Riming, Dong, Jianghui, Jin, Ling
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.06.2020; John Wiley and Sons Inc
• Subjects: Acanthaceae - chemistry; Adenosine kinase; AMP; AMP-Activated Protein Kinases - metabolism; AMPK/ Nrf2; Analgesics; Animals; Calories; Dicliptera chinensis; Dicliptera chinensis polysaccharides; Ethanol; Glucose metabolism; Hep G2 Cells; Humans; Inflammation; Laboratory animals; Lipid metabolism; Lipid Metabolism - drug effects; Liver; Liver - drug effects; Liver - metabolism; Liver - pathology; Liver - physiopathology; Liver diseases; liver disturbances; Male; Mice, Inbred C57BL; Models, Biological; NF-E2-Related Factor 2 - metabolism; NF-kappa B - metabolism; Original; Oxidative metabolism; Oxidative stress; Oxidative Stress - drug effects; Peroxisome proliferator-activated receptors; Polysaccharides; Polysaccharides - pharmacology; Protein expression; Protein kinase; Proteins; Signal transduction; Signal Transduction - drug effects; siRNA; Sterol regulatory element-binding protein; TLR‐4/ NF‐κB; Toll-Like Receptor 4 - metabolism; Toll-like receptors; Tumor necrosis factor; Tumors; Beijing China; China; AMPK/ Nrf2; Dicliptera chinensis polysaccharides; TLR-4/ NF-κB; liver disturbances
• ISSN: 1582-1838; 1582-4934
• DOI: 10.1111/jcmm.15286

The purpose of this study was to alleviate liver disturbance by applying polysaccharides from Dicliptera chinensis (DCP) to act on the adenosine monophosphate–activated protein kinase/ nuclear factor erythroid 2‐related factor 2 (AMPK/ Nrf2) oxidative stress pathway and the Toll‐like receptor 4 (TLR‐4)/ nuclear factor kappa‐B (NF‐κB) inflammatory pathway and to establish an in vivo liver disturbance model using male C57BL/6J and TLR‐4 knockout (−/−) mice. For this, we evaluated the expression levels of SREBP‐1 and Nrf2 after silencing the expression of AMPK using siRNA technology. Our results show that with regard to the TLR‐4/ NF‐κB inflammatory pathway, DCP inhibits TLR‐4, up‐regulates the expression of peroxisome proliferator‐activated receptor‐γ (PPAR‐γ), reduces the expression of phospho(p)‐NF‐κB and leads to the reduction of downstream inflammatory factors, such as tumour necrosis factor‐α (TNF‐α), interleukin (IL)‐6 and IL‐1β, thereby inhibiting the inflammatory response. Regarding the AMPK/ Nrf2 oxidative stress pathway, DCP up‐regulates the expression of p‐AMPK and Nrf2, in addition to regulating glucose and lipid metabolism, oxidative stress and ameliorating liver disturbance symptoms. In summary, our study shows that DCP alleviates liver disturbances by inhibiting mechanisms used during liver inflammation and oxidative stress depression, which provides a new strategy for the clinical treatment of liver disturbance.