Streptococcus mutans Inhibits Candida albicans Hyphal Formation by the Fatty Acid Signaling Molecule trans-2-Decenoic Acid (SDSF)

• Published in: Chembiochem : a European journal of chemical biology Vol. 11; no. 11; pp. 1552 - 1562
• Main Authors: Vílchez, Ramiro, Lemme, André, Ballhausen, Britta, Thiel, Verena, Schulz, Stefan, Jansen, Rolf, Sztajer, Helena, Wagner-Döbler, Irene
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 26.07.2010; WILEY‐VCH Verlag
• Subjects: Biofilms; Candida albicans; Candida albicans - ultrastructure; Fatty Acids - pharmacology; Fatty Acids, Monounsaturated - pharmacology; Humans; Hyphae - drug effects; Hyphae - growth & development; luciferase; metabolism; Mouth - microbiology; quorum sensing; Signal Transduction; Streptococcus mutans - physiology
• ISSN: 1439-4227; 1439-7633
• DOI: 10.1002/cbic.201000086

In the human mouth, fungi and several hundred species of bacteria coexist. Here we report a case of interkingdom signaling in the oral cavity: A compound excreted by the caries bacterium Streptococcus mutans inhibits the morphological transition from yeast to hyphae, an important virulence trait, in the opportunistic fungus Candida albicans. The compound excreted by S. mutans was originally studied because it inhibited signaling by the universal bacterial signal autoinducer‐2 (AI‐2), determined by the luminescence of a Vibrio harveyi sensor strain. The inhibitor was purified from cell‐free culture supernatants of S. mutans guided by its activity. Its chemical structure was elucidated by using NMR spectroscopy and GC‐MS and proved to be trans‐2‐decenoic acid. We show that trans‐2‐decenoic acid does not inhibit AI‐2‐specific signaling, but rather the luciferase reaction used for its detection. A potential biological role of trans‐2‐decenoic acid was then discovered. It is able to suppress the transition from yeast to hyphal morphology in the opportunistic human pathogen Candida albicans at concentrations that do not affect growth. The expression of HWP1, a hyphal‐specific signature gene of C. albicans, is abolished by trans‐2‐decenoic acid. trans‐2‐Decenoic acid is structurally similar to the diffusible signal factor (DSF) family of interkingdom‐signaling molecules and is the first member of this family from a Gram‐positive organism (Streptococcus DSF, SDSF). SDSF activity was also found in S. mitis, S. oralis, and S. sanguinis, but not in other oral bacteria. SDSF could be relevant in shaping multispecies Candida bacteria biofilms in the human body. Flora and fauna at war: We report a case of interkingdom signaling in the oral cavity. The caries bacterium Streptococcus mutans excretes trans‐2‐decenoic acid, a diffusible signal factor that inhibits filamentation of the opportunistic fungus Candida albicans. This morphological transition is important for virulence and biofilm formation.