Systematic interpolation method predicts protein chromatographic elution with salt gradients, pH gradients and combined salt/pH gradients

• Published in: Biotechnology journal Vol. 12; no. 3
• Main Authors: Creasy, Arch, Barker, Gregory, Carta, Giorgio
• Format: Journal Article
• Language: English
• Published: Germany 01.03.2017
• Subjects: Antibodies, Monoclonal - chemistry; Buffers; Cation Exchange Resins; Chromatography, Ion Exchange; Hydrogen-Ion Concentration; Models, Chemical; Protein Binding; Proteins - chemistry; Proteins - isolation & purification; Proton-Motive Force - physiology; Sensitivity and Specificity; Sodium Chloride - chemistry; Proteins; Linear salt gradients; Linear pH gradients; Predictive modeling; Ion exchange chromatography
• ISSN: 1860-7314
• DOI: 10.1002/biot.201600636

A methodology is presented to predict protein elution behavior from an ion exchange column using both individual or combined pH and salt gradients based on high-throughput batch isotherm data. The buffer compositions are first optimized to generate linear pH gradients from pH 5.5 to 7 with defined concentrations of sodium chloride. Next, high-throughput batch isotherm data are collected for a monoclonal antibody on the cation exchange resin POROS XS over a range of protein concentrations, salt concentrations, and solution pH. Finally, a previously developed empirical interpolation (EI) method is extended to describe protein binding as a function of the protein and salt concentration and solution pH without using an explicit isotherm model. The interpolated isotherm data are then used with a lumped kinetic model to predict the protein elution behavior. Experimental results obtained for laboratory scale columns show excellent agreement with the predicted elution curves for both individual or combined pH and salt gradients at protein loads up to 45 mg/mL of column. Numerical studies show that the model predictions are robust as long as the isotherm data cover the range of mobile phase compositions where the protein actually elutes from the column.