Miniaturized Molecularly Imprinted Continuous Polymer Rods

• Published in: Journal of high resolution chromatography Vol. 23; no. 1; pp. 44 - 46
• Main Authors: Takeuchi, Toshifumi, Matsui, Jun
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.01.2000; Wiley-VCH
• Subjects: Analytical chemistry; Chemistry; chiral recognition; Chromatographic methods and physical methods associated with chromatography; continuous polymer rod; diastereoselectivity; Exact sciences and technology; In situ molecular imprinting; miniaturized column; Other chromatographic methods; Capillary column; Internal lining; Acrylic acid copolymer; Alkaloid; Ultraviolet detector; Separation; Optical resolution; Enantiomer; Preparation; HPLC chromatography; Methacrylate copolymer; Experimental study
• ISSN: 0935-6304; 1521-4168
• DOI: 10.1002/(SICI)1521-4168(20000101)23:1<44::AID-JHRC44>3.0.CO;2-6

Molecularly imprinted continuous polymer rods are prepared inside PEEK tubes of 1 mm i. d. by an in situ preparation technique; and chiral separations on such media have been demonstrated. The miniaturized column filled with (–)‐cinchonidine‐imprinted or (+)‐cinchonine‐imprinted polymer rods based on a copolymer of 2‐(trifluoromethyl)acrylic acid and ethylene glycol dimethacrylate permitted diastereoseparation of (–)‐cinchonidine and (+)‐cinchonine; the template molecule is retained to a greater extent than the corresponding antipode. Increasing the amount of cyclohexanol in the polymerization mixture decreased the backpressure of the resultant rod and the addition of latex beads suspended in water to the prepolymerization mixture affected the selectivity and affinity of the imprinted polymer rods. The column length is adjusted easily by cutting, thus allowing easy control of retention behavior and analysis time.