A Near-Infrared Fluorescent Nanosensor (AuC@Urease) for the Selective Detection of Blood Urea

The use of a nanosensor (AuC@Urease) based on an NIR‐emitting gold cluster and urease enzyme selectively detects urea in whole blood. The detection is based on an enzyme‐specific conversion of urea to ammonium ions which facilitates pH‐induced aggregation of AuC, leading to fluorescence quenching. T...

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Published inSmall (Weinheim an der Bergstrasse, Germany) Vol. 9; no. 16; pp. 2673 - 2677
Main Authors Nair, Lakshmi V., Philips, Divya S., Jayasree, Ramapurath S., Ajayaghosh, Ayyappanpillai
Format Journal Article
LanguageEnglish
Published Weinheim WILEY-VCH Verlag 26.08.2013
WILEY‐VCH Verlag
Wiley Subscription Services, Inc
Subjects
Biosensing Techniques - methods
gold quantum clusters
Humans
nanobiosensors
Nanotechnology
Spectroscopy, Near-Infrared - methods
Urea - blood
urea sensors
urease
Urease - chemistry
Urease - metabolism
whole blood analysis
whole blood analysis
gold quantum clusters
nanobiosensors
urea sensors
urease
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Summary:The use of a nanosensor (AuC@Urease) based on an NIR‐emitting gold cluster and urease enzyme selectively detects urea in whole blood. The detection is based on an enzyme‐specific conversion of urea to ammonium ions which facilitates pH‐induced aggregation of AuC, leading to fluorescence quenching. This method does not interfere with urease inactive analytes or the autofluorescence of blood as confirmed by comparable urea levels obtained by AuC@Urease and standard clinical methods within an error limit of ±3%.
Bibliography:ark:/67375/WNG-9LVK7GLX-2
istex:E3927EB9B88D8C5576F305DF64D9DEF32DCD7ACA
ArticleID:SMLL201300213
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1613-6810
1613-6829
DOI:10.1002/smll.201300213