Transcriptome wide analysis of long non‐coding RNA‐associated ceRNA regulatory circuits in psoriasis

• Published in: Journal of cellular and molecular medicine Vol. 25; no. 14; pp. 6925 - 6935
• Main Authors: Lin, Jingxia, Li, Xuefei, Zhang, Fangfei, Zhu, Lei, Chen, Yongfeng
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.07.2021; John Wiley and Sons Inc
• Subjects: Autoimmune diseases; Binding sites; Biopsy; ceRNA; Cytokines; Data analysis; Datasets; Dendritic cells; Dermatitis; Disease; Gene expression; Gene Regulatory Networks; Genomes; HaCaT Cells; Humans; IFN gamma signalling pathway; Inflammation; Inflammatory diseases; Interferon; Interferon-gamma - genetics; Interferon-gamma - metabolism; JAK/STAT pathway; Janus Kinases - genetics; Janus Kinases - metabolism; Kinases; long non‐coding RNAs; MicroRNAs; MicroRNAs - genetics; MicroRNAs - metabolism; miRNA; Non-coding RNA; Original; Pathogenesis; Physiology; Proteins; Psoriasis; Psoriasis - genetics; Psoriasis - metabolism; Ribonucleic acid; RNA; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; RNA‐seq; Signal Transduction; Skin - metabolism; Skin diseases; Stat1 protein; STAT1 Transcription Factor - genetics; STAT1 Transcription Factor - metabolism; Transcription activation; Transcriptome; Transcriptomes; ceRNA; RNA-seq; long non-coding RNAs; JAK/STAT pathway; IFN gamma signalling pathway
• ISSN: 1582-1838; 1582-4934
• DOI: 10.1111/jcmm.16703

Long non‐coding RNAs (lncRNAs) play critical roles in regulating immune‐associated diseases and chronic inflammatory disorders. Here, we found that lncRNAs involve in the pathogenesis of psoriasis through integrative analysis of RNA‐seq data sets from a psoriasis cohort. Then, lncRNA‐protein‐coding genes (PCGs) co‐expression network analysis demonstrated that lncRNAs extensively interact with IFN‐γ signalling pathway‐associated genes. Further, we validated 3 lncRNAs associate with IFN‐γ signalling pathway activation upon IFN‐γ stimulated in HaCaT cells, and loss of function experiments indicate their functional roles in the activation of inflammatory cytokine genes. Additionally, microRNA target screening analysis showed that lncRNAs may regulate JAK/STAT pathway activity through complete endogenous RNA (ceRNA) mechanism. Further experimental validation of PRKCQ‐AS1/STAT1/miR‐545‐5p regulatory circuitry showed that lncRNAs regulate the expression of JAK/STAT signalling pathway genes through competing for miR‐545‐5p. In summary, our results demonstrated that dysregulation of lncRNA‐JAK/STAT pathway axis promotes the inflammation level in psoriasis and thus provide potential therapeutic targets for psoriasis treatments.