Computer analysis of two-dimensional electrophoresis gels: a new segmentation and modeling algorithm

The complexity of the spot patterns of two-dimensional electrophoresis gels made it necessary to use image processing techniques to analyze the gels. An important issue in the analysis is the detection and quantification of the protein spots. In this paper we describe a new technique to segment and...

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Bibliographic Details
Published inElectrophoresis Vol. 18; no. 5; p. 792
Main Authors Bettens, E, Scheunders, P, Van Dyck, D, Moens, L, Van Osta, P
Format Journal Article
LanguageEnglish
Published Germany 1997
Subjects
Algorithms
Electrophoresis, Gel, Two-Dimensional - methods
Gels
Image Processing, Computer-Assisted
Models, Statistical
Proteins - analysis
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Summary:The complexity of the spot patterns of two-dimensional electrophoresis gels made it necessary to use image processing techniques to analyze the gels. An important issue in the analysis is the detection and quantification of the protein spots. In this paper we describe a new technique to segment and model the different spots. For the segmentation of a gel into its different spot regions we apply a watershed technique, which is robust and efficient. For the quantification of the spots, a new spot model is constructed, based on diffusion principles. Besides the advantage of having a physical interpretation, the model is demonstrated to be superior to the commonly used Gaussian models.
ISSN:0173-0835
DOI:10.1002/elps.1150180523