HN1L promotes migration and invasion of breast cancer by up‐regulating the expression of HMGB1

• Published in: Journal of cellular and molecular medicine Vol. 25; no. 1; pp. 397 - 410
• Main Authors: Jiao, Dechuang, Zhang, Jingyang, Chen, Ping, Guo, Xuhui, Qiao, Jianghua, Zhu, Jiujun, Wang, Lina, Lu, Zhenduo, Liu, Zhenzhen
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.01.2021; John Wiley and Sons Inc
• Subjects: Antibodies; Bioinformatics; Blotting, Western; Breast cancer; Breast Neoplasms - genetics; Breast Neoplasms - metabolism; Cell culture; Cell cycle; Cell growth; Cell Movement - genetics; Cell Movement - physiology; Cell Proliferation - genetics; Cell Proliferation - physiology; Hematology; HMGB1; HMGB1 protein; HMGB1 Protein - genetics; HMGB1 Protein - metabolism; HN1L; HSPA9; Humans; Immunofluorescence; Immunohistochemistry; Immunoprecipitation; invasion and metastasis; MCF-7 Cells; Medical prognosis; Metastases; Metastasis; Microtubule-Associated Proteins - genetics; Microtubule-Associated Proteins - metabolism; Original; Ovaries; Patients; Plasmids; Prostate; Proteins; Real-Time Polymerase Chain Reaction; Survival analysis; Tandem Mass Spectrometry; Therapeutic targets; Tumorigenesis; Tumors; Wound healing; Wound Healing - genetics; Wound Healing - physiology; HN1L; HSPA9; breast cancer; HMGB1; invasion and metastasis
• ISSN: 1582-1838; 1582-4934
• DOI: 10.1111/jcmm.16090

Recent reports showed that haematological and neurological expressed 1‐like (HN1L) gene participated in tumorigenesis and tumour invasion. However, the expression and role of HN1L in breast cancer remain to be investigated. Here, bioinformatics, western blot and immunohistochemistry were used to detect the expression of HN1L in breast cancer. Wound healing, transwell assay, immunofluorescence assay and mass spectrum were used to explore the role and mechanism of HN1L on the migration and invasion of breast cancer, which was confirmed in vivo using a nude mice model. Results showed that HN1L was significantly over‐expressed in breast cancer tissues, which was positively correlated with M metastasis of breast cancer patients. Silencing HN1L significantly inhibited the invasion and metastasis of breast cancer cells in vitro and lung metastasis in nude mice metastasis model of breast cancer. Mechanistically, HN1L interacted with HSPA9 and affected the expression of HMGB1, playing a key role in promoting the invasion and metastasis of breast cancer cell. These results suggested that HN1L was an appealing drug target for breast cancer.