Toehold-controlled ligation and transcription for accurate COVID-19 genotyping

• Published in: Analytical biochemistry Vol. 654; p. 114803
• Main Authors: Gao, Yanmin, Wang, Taoxue, Li, Jiaojiao, Wei, Yanan, Qi, Hao
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.10.2022
• Subjects: Clinical Laboratory Techniques - methods; COVID-19; COVID-19 - diagnosis; COVID-19 Testing; Genotype; Humans; Ligase chain reaction; Nucleic Acid Amplification Techniques - methods; SARS-CoV-2; SARS-CoV-2 - genetics; Sensitivity and Specificity; Toehold-mediated strand displacement reaction; COVID-19; SARS-CoV-2; Toehold-mediated strand displacement reaction; Ligase chain reaction
• ISSN: 0003-2697; 1096-0309; 1096-0309
• DOI: 10.1016/j.ab.2022.114803

The global pandemic of coronavirus disease 2019 (COVID-19) has significant impact on the entire human society. However, in the face of continually emerging more contagious SARS-CoV-2 variant, the risk to bog down into more severe crisis is around us anytime. Here, we introduce an isothermal, ultrasensitive method for identifying important SNV mutations of SARS-CoV-2. It is based on combined specificity of toehold-assisted linear probe ligation and in vitro transcription signal enlargement, TLT. A ready-to-use panel of TLT assay is developed including detection of 80 crucial SARS-CoV-2 SNVs, by which people could response to the next coming contagious virus variant more rapidly. These advanced point-of-care features make TLT one good approach for large scale population testing of special SARS-CoV-2 variants of interesting. An isothermal, ultrasensitive method for identifying SNV mutations of SARS-CoV-2, which based on combined specificity of toehold-assisted linear probe ligation and in vitro transcription signal enlargement, TLT. A ready-to-use panel of TLT assay is developed including detection of 80 crucial SARS-CoV-2 SNVs. [Display omitted] •An isothermal, ultrasensitive method for identifying SNV mutations of SARS-CoV-2.•Toehold-assisted linear probe ligation and in vitro transcription signal enlargement, TLT.•Detection of 80 crucial SARS-CoV-2 SNVs.