Establishment and characterization of human hepatocellular carcinoma cell line FHCC-98

• Published in: World journal of gastroenterology : WJG Vol. 10; no. 10; pp. 1462 - 1465
• Main Authors: Lou, Chao-Yang, Feng, Ying-Ming, Qian, Ai-Rong, Li, Yu, Tang, Hao, Shang, Peng, Chen, Zhi-Nan
• Format: Journal Article
• Language: English
• Published: United States Department of Oncology,Tangdu Hospital, Fourth Military Medical University, Xi'an 710038,Shaanxi Province, China%Department of Cell Biology, Fourth Military Medical University,Xi'an 710032, Shaanxi Province, China 15.05.2004; Baishideng Publishing Group Inc
• Subjects: Adult; Animals; Basic Research; Biomarkers, Tumor; Carcinoma, Hepatocellular - metabolism; Carcinoma, Hepatocellular - pathology; Cell Culture Techniques - methods; Cell Line, Tumor - cytology; Cell Line, Tumor - metabolism; Cell Shape; FHCC-98细胞; Humans; Liver Neoplasms - metabolism; Liver Neoplasms - pathology; Male; Mice; Mice, Nude; Neoplasm Transplantation; Transplantation, Heterologous; 肝细胞癌; 肿瘤生物学; 肿瘤转移
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v10.i10.1462

AIM: To establish a novel human hepatocellular carcinoma (HCC) cell line FHCC-98 from HCC tissue and to provide a suitable model for studying HCC occurrence, progress and metastasis.METHODS: Serially passaged cells were cultured and their morphologies were observed under light and electron microscope. Cytogenetic study was conducted by using flow cytometry and chromosome analysis. Expressions of tumor markers such as α-fetoprotein (AFP), cytokeratin (CK) and hepatoma metastasis-associated factor HAb18G/CD147 on the FHCC-98 cells were detected by immunocytochemistry or Western blotting. Lactic dehydrogenase (LDH) isoenzymes were detected by polyacrylamide gel electrophoresis (PAGE).Xenograft was performed by inoculating FHCC-98 cells into the flanks of nude mice.RESULTS: Morphology of FHCC-98 cells was the same as that of other malignant cells. The expressions of the cells were positive for HAb18G/CD147 and CK, and negative for AFP. Its population doubling time was 21.4 h. The cell DNA was tetraploid and the major chromosomes were triploid by cytogenetics analysis. The tumorigenicity in nude mice was 100%. PAGE showed four bands representing LDH2, LDH3,LDH4 and LDH5.CONCLUSION: FHCC-98 is a novel HCC cell line and an ideal cell model for further exploring the mechanism of hepatocellular carcinoma invasion and metastasis.