An EIS study of DNA-modified electrodes

The preparation and conditioning of DNA-modified electrodes, in which glassy carbon electrodes are covered by a thick film of double-stranded DNA and subsequently conditioned in acetate buffer, has been followed with Electrochemical Impedance Spectroscopy. Spectra were recorded at different values o...

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Bibliographic Details
Published inElectrochimica acta Vol. 44; no. 24; pp. 4233 - 4239
Main Authors Brett, Christopher M.A., Oliveira Brett, Ana Maria, Serrano, Silvia H.P.
Format Journal Article
LanguageEnglish
Published Elsevier Ltd 01.01.1999
Subjects
Adenine
Biosensors
Conductive materials
DNA
DNA-modified electrodes
Double-stranded DNA
Electrochemical impedance
Glass
Guanine
Probes
Single-stranded DNA
Solutions
Spectroscopic analysis
Surface phenomena
Adenine
Double-stranded DNA
DNA-modified electrodes
Electrochemical impedance
Single-stranded DNA
Guanine
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Summary:The preparation and conditioning of DNA-modified electrodes, in which glassy carbon electrodes are covered by a thick film of double-stranded DNA and subsequently conditioned in acetate buffer, has been followed with Electrochemical Impedance Spectroscopy. Spectra were recorded at different values of applied potential in order to probe the alterations occurring in the modifier layer. The alterations in the EIS response parallel the changes in voltammetric response and show that the modifier layer is a good conductor and that the behaviour as a DNA-biosensor is essentially due to surface phenomena and interaction with single-stranded DNA in solution during conditioning.
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ISSN:0013-4686
1873-3859
DOI:10.1016/S0013-4686(99)00138-3