A Multifunctionalized Potyvirus-Derived Nanoparticle That Targets and Internalizes into Cancer Cells

Plant viral nanoparticles (VNPs) are attractive to nanomedicine researchers because of their safety, ease of production, resistance, and straightforward functionalization. In this paper, we developed and successfully purified a VNP derived from turnip mosaic virus (TuMV), a well-known plant pathogen...

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Bibliographic Details
Published inInternational journal of molecular sciences Vol. 25; no. 8; p. 4327
Main Authors Truchado, Daniel A, Juárez-Molina, María, Rincón, Sara, Zurita, Lucía, Tomé-Amat, Jaime, Lorz, Corina, Ponz, Fernando
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 01.04.2024
Subjects
Amino acids
Antibodies
Binding sites
Cancer research
Cancer therapies
Cell Line, Tumor
cetuximab
Cetuximab - chemistry
Cetuximab - pharmacology
Drugs
Epidermal growth factor
Ethylenediaminetetraacetic acid
Flow cytometry
Humans
Immunoglobulin G
Immunoglobulin G - metabolism
Immunoglobulins
Medical research
Metastasis
Microscopy
Nanoparticles - chemistry
Potyvirus
protein A
Proteins
Scientific equipment and supplies industry
Squamous cell carcinoma
Staphylococcal Protein A - chemistry
Staphylococcal Protein A - metabolism
Tongue
turnip mosaic virus
Viruses
VLP
Z domain
United States
Massachusetts
Germany
Spain
turnip mosaic virus
cetuximab
protein A
VLP
viral nanoparticles
Z domain
squamous cell carcinoma
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Summary:Plant viral nanoparticles (VNPs) are attractive to nanomedicine researchers because of their safety, ease of production, resistance, and straightforward functionalization. In this paper, we developed and successfully purified a VNP derived from turnip mosaic virus (TuMV), a well-known plant pathogen, that exhibits a high affinity for immunoglobulins G (IgG) thanks to its functionalization with the Z domain of staphylococcal Protein A via gene fusion. We selected cetuximab as a model IgG to demonstrate the versatility of this novel TuMV VNP by developing a fluorescent nanoplatform to mark tumoral cells from the Cal33 line of a tongue squamous cell carcinoma. Using confocal microscopy, we observed that fluorescent VNP-cetuximab bound selectively to Cal33 and was internalized, revealing the potential of this nanotool in cancer research.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms25084327