IMPROVED METHODS FOR THE PREPARATION OF THE SPECIFIC POLYSACCHARIDES OF PNEUMOCOCCUS

The specific polysaccharides of Types I and V pneumococcus give sharp equivalence zones and show maximal precipitation with homologous rabbit antisera only when carefully prepared from cultures which have not been neutralized with alkali. The precipitating power of S V toward homologous rabbit antis...

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Published inThe Journal of experimental medicine Vol. 91; no. 4; pp. 341 - 349
Main Authors Heidelberger, M, Macleod, C M, Markowitz, H, Roe, A S
Format Journal Article
LanguageEnglish
Published United States The Rockefeller University Press 01.04.1950
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Summary:The specific polysaccharides of Types I and V pneumococcus give sharp equivalence zones and show maximal precipitation with homologous rabbit antisera only when carefully prepared from cultures which have not been neutralized with alkali. The precipitating power of S V toward homologous rabbit antiserum falls off in 0.1 N NaOH even more rapidly than that of S I, dropping to 7 per cent of the original value in 6 days at room temperature. The alkaline solution develops a large absorption peak at 270 mmicro. Directions are given for the preparation of S VII and S XII.
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ISSN:0022-1007
1540-9538
DOI:10.1084/jem.91.4.341