Identification of novel immunogenic proteins in pathogenic Haemophilus parasuis based on genome sequence analysis
Haemophilus parasuis causes contagious porcine Glässer's disease, which is occurring worldwide and leads to severe losses in the pig industry. To identify novel antigen candidates against this disease, 22 surface-exposed or secreted proteins were selected from the annotated H. parasuis genome b...
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Published in | Veterinary microbiology Vol. 148; no. 1; pp. 89 - 92 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Amsterdam
Elsevier B.V
24.02.2011
Amsterdam; New York: Elsevier Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | Haemophilus parasuis causes contagious porcine Glässer's disease, which is occurring worldwide and leads to severe losses in the pig industry. To identify novel antigen candidates against this disease, 22 surface-exposed or secreted proteins were selected from the annotated
H. parasuis genome by reverse vaccinology strategy. Expression of these proteins in
Escherichia coli was attempted. Immunogenicity of the expressed candidates was assessed using Western blot analysis with mouse-derived antiserum prepared with whole bacteria of
H. parasuis serovar 4 or 5. Three ABC-type transporters (OppA, YfeA and PlpA) and 1 curli protein assembly (CsgG) were identified as potent immunogenic proteins. The proteins show cross-reactions when tested with sera raised against serovars 4 and 5 of
H. parasuis. |
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Bibliography: | http://dx.doi.org/10.1016/j.vetmic.2010.07.023 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1 |
ISSN: | 0378-1135 1873-2542 |
DOI: | 10.1016/j.vetmic.2010.07.023 |