Identification of novel immunogenic proteins in pathogenic Haemophilus parasuis based on genome sequence analysis

• Published in: Veterinary microbiology Vol. 148; no. 1; pp. 89 - 92
• Main Authors: Hong, Minhee, Ahn, Jungoh, Yoo, Sungsik, Hong, Jiyeon, Lee, Eungyu, Yoon, Injoong, Jung, Joon-ki, Lee, Hongweon
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 24.02.2011; Amsterdam; New York: Elsevier; Elsevier
• Subjects: Animals; Antigenic protein; Bacterial Proteins - genetics; Bacterial Proteins - immunology; Bacteriology; Biological and medical sciences; Cross Reactions; DNA, Bacterial - genetics; Escherichia coli; Escherichia coli - genetics; Fundamental and applied biological sciences. Psychology; Genes, Bacterial; Genome, Bacterial; Haemophilus parasuis; Haemophilus parasuis - genetics; Haemophilus parasuis - immunology; Immune Sera - immunology; Mice; Microbiology; Miscellaneous; Reverse vaccinology; Reverse vaccinology; Antigenic protein; Haemophilus parasuis; Pasteurellaceae; Haemophilus; Immunogenicity; Nucleotide sequence; Bacteria; Identification; Veterinary; Protein
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/j.vetmic.2010.07.023

Haemophilus parasuis causes contagious porcine Glässer's disease, which is occurring worldwide and leads to severe losses in the pig industry. To identify novel antigen candidates against this disease, 22 surface-exposed or secreted proteins were selected from the annotated H. parasuis genome by reverse vaccinology strategy. Expression of these proteins in Escherichia coli was attempted. Immunogenicity of the expressed candidates was assessed using Western blot analysis with mouse-derived antiserum prepared with whole bacteria of H. parasuis serovar 4 or 5. Three ABC-type transporters (OppA, YfeA and PlpA) and 1 curli protein assembly (CsgG) were identified as potent immunogenic proteins. The proteins show cross-reactions when tested with sera raised against serovars 4 and 5 of H. parasuis.