Purification of reverse transcriptase from avian retroviruses using affinity chromatography on heparin-sepharose

Reverse transcriptase from Rous sarcoma virus and avian myeloblastosis virus was purified by a rapid two-step procedure using chromatography on phosphocellulose and heparin-Sepharose. The resulting enzyme was homogeneous, had a high specific activity and was free of contaminating nucleases. This pro...

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Bibliographic Details
Published inJournal of virological methods Vol. 1; no. 3; pp. 157 - 165
Main Authors Golomb, M, Vora, A.C, Grandgenett, D.P
Format Journal Article
LanguageEnglish
Published Netherlands 01.01.1980
Subjects
animal diseases
animal health
Avian Leukosis Virus - enzymology
Avian Myeloblastosis Virus - enzymology
Avian Sarcoma Viruses - enzymology
Chemical Phenomena
Chemistry
Chromatography, Affinity
Heparin
Methods
RNA-Directed DNA Polymerase - isolation & purification
Sepharose
viral diseases of animals and humans
Viral Proteins
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Summary:Reverse transcriptase from Rous sarcoma virus and avian myeloblastosis virus was purified by a rapid two-step procedure using chromatography on phosphocellulose and heparin-Sepharose. The resulting enzyme was homogeneous, had a high specific activity and was free of contaminating nucleases. This procedure has been adapted to small-scale preparation of enzyme from mutant virus containing thermolabile reverse transcriptase, and is equally suitable for large-scale enzyme purification.
ISSN:0166-0934
1879-0984
DOI:10.1016/0166-0934(80)90012-9