LncRNA MIR155HG functions as a ceRNA of miR-223-3p to promote cell pyroptosis in human degenerative NP cells

• Published in: Clinical and experimental immunology Vol. 207; no. 2; pp. 241 - 252
• Main Authors: Yang, Wei, Huang, Xu-Dong, Zhang, Tao, Zhou, You-Bin, Zou, Yong-Cheng, Zhang, Jian
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 04.04.2022
• Subjects: Humans; Intervertebral Disc Degeneration - genetics; Intervertebral Disc Degeneration - metabolism; Intervertebral Disc Degeneration - pathology; MicroRNAs - genetics; Nucleus Pulposus - metabolism; Nucleus Pulposus - pathology; Pyroptosis - genetics; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; MIR155HG; IDD; ceRNA; pyroptosis; NLRP3; miR-223-3p
• ISSN: 0009-9104; 1365-2249
• DOI: 10.1093/cei/uxab030

Nucleus pulposus (NP) cell pyroptosis plays a critical role in the pathogenesis of intervertebral disk degeneration (IDD). MIR155 host gene (MIR155HG) is a long non-coding RNA with pro-inflammatory activity. However, very little is known about its role in NP cell pyroptosis. This study aimed to observe the impact of MIR155HG on cell pyroptosis and to explore the underlying mechanism in human degenerative NP cells. Our results demonstrated that MIR155HG expression was significantly increased in human degenerative NP tissue samples and showed a positive correlation with Pfirrmann score. Overexpression of MIR155HG through a lentiviral vector decreased miR-223-3p levels, up-regulated NLRP3 expression and induced cell pyroptosis in human degenerative NP cells. A ceRNA action mode was identified among MIR155HG, miR-223-3p, and NLRP3. The stimulatory effect of MIR155HG on human degenerative NP cell pyroptosis was significantly reversed by pretreatment with miR-223-3p mimic or NLRP3 siRNA. In summary, these data suggest that MIR155HG sponges miR-223-3p to promote NLRP3 expression, leading to induction of cell pyroptosis in human degenerative NP cells. Targeting MIR155HG could be a novel and promising strategy to slow down the progression of IDD.