New sites of cellular vitronectin receptor immunoreactivity detected with osteoclast-reacting monoclonal antibodies 13C2 and 23C6

• Published in: Bone and mineral Vol. 8; no. 1; pp. 7 - 22
• Main Authors: Athanasou, N.A., Quinn, J., Horton, M.A., McGee, J.O'D.
• Format: Journal Article
• Language: English
• Published: London Elsevier Inc 1990; Amsterdam Elsevier; New York, NY
• Subjects: Antibodies, Monoclonal; Antigen-antibody reactions, antigen-antibody complexes, antibody-complement and others. Study of affinity. Antigen presentation; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Giant cell; Humans; Immunoenzyme Techniques; Macrophage; Macrophages - analysis; Molecular immunology; Monoclonal antibody; Organ Specificity; Osteoclast; Osteoclasts - analysis; Receptors, Immunologic - analysis; Receptors, Vitronectin; Osteoclast; Monoclonal antibody; Macrophage; Giant cell; Antigen; Human; Immunohistochemistry; Antigenic determinant; Immunogenicity; Cross reaction; Antigen antibody reaction; Biological receptor
• ISSN: 0169-6009
• DOI: 10.1016/0169-6009(91)90136-N

The immunohistochemical profile of osteoclast-reacting monoclonal antibodies, 13C2 and 23C6, known to detect the α-chain of the vitronectin receptor, is described. Both antibodies reacted with several cell types apart from osteoclasts including megakaryocytes, smooth muscle cells, endothelial cells, thyroid follicular epithelium, renal glomeruli and tubular epithelium, myoepithelial and epithelial cells in the breast and prostate, and both cytotrophoblast and syncytiotrophoblast. In addition, macrophage polykaryons, synovial lining cells, a small number of mononuclear cells in buffy coats, and a few macrophage-like cells in the stroma of various tissues were also stained. The epitopes recognized by these antibodies are thus not osteoclast-specific and are present on other cells of the mononuclear phagocyte system. The implications of these results for osteoclast ontogeny, the nature of the antigens described and the question of osteoclast-specific antibodies are discussed.