The Photoperiodic Flowering Time Regulator FKF1 Negatively Regulates Cellulose Biosynthesis

Cellulose synthesis is precisely regulated by internal and external cues, and emerging evidence suggests that light regulates cellulose biosynthesis through specific light receptors. Recently, the blue light receptor CRYPTOCHROME 1 (CRY1) was shown to positively regulate secondary cell wall biosynth...

Full description

Saved in:
Bibliographic Details
Published inPlant physiology (Bethesda) Vol. 180; no. 4; pp. 2240 - 2253
Main Authors Yuan, Ning, Balasubramanian, Vimal Kumar, Chopra, Ratan, Mendu, Venugopal
Format Journal Article
LanguageEnglish
Published United States American Society of Plant Biologists 01.08.2019
Subjects
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Cryptochromes - genetics
Cryptochromes - metabolism
Flowers - genetics
Flowers - metabolism
Gene Expression Regulation, Plant
Glucosyltransferases - genetics
Glucosyltransferases - metabolism
Photoperiod
Point Mutation - genetics
Online AccessGet full text

Cover

More Information
Summary:Cellulose synthesis is precisely regulated by internal and external cues, and emerging evidence suggests that light regulates cellulose biosynthesis through specific light receptors. Recently, the blue light receptor CRYPTOCHROME 1 (CRY1) was shown to positively regulate secondary cell wall biosynthesis in Arabidopsis ( ). Here, we characterize the role of , ( ), another blue light receptor and well-known photoperiodic flowering time regulator, in cellulose biosynthesis. A phenotype suppression screen using a cellulose deficient mutant ( ), which carries nonlethal point mutations in ( ) and , resulted in identification of the phenotype-restoring ( ) mutant. Next-generation mapping using the whole genome resequencing method identified the locus as was confirmed as the causal gene through observation of the phenotype in an independent triple mutant carrying a T-DNA insertion mutant. Moreover, overexpression of in plants restored the phenotype. The mutants showed significant increases in cellulose content and gene expression compared with that in wild-type Columbia-0 plants, suggesting a negative role of FKF1 in cellulose biosynthesis. Using genetic, molecular, and phenocopy and biochemical evidence, we have firmly established the role of FKF1 in regulation of cellulose biosynthesis. In addition, expression analysis showed that diurnal expression patterns of s are FKF1 independent, whereas their circadian expression patterns are FKF1 dependent. Overall, our work establishes a role of FKF1 in the regulation of cell wall biosynthesis in Arabidopsis.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Senior author.
www.plantphysiol.org/cgi/doi/10.1104/pp.19.00013
N.Y. performed molecular, genetic, phenocopy, and gene expression analysis; N.Y. and V.K.B. wrote the first draft; N.Y., V.K.B., and R.C. edited the article; V.K.B. generated mutants and mapping population and performed biochemical analysis; R.C. performed bioinformatic analysis; V.M. conceived the idea and designed experiments; all the authors read and approved the final draft.
The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Venugopal Mendu (venugopal.mendu@ttu.edu).
These authors contributed equally to the article.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.19.00013