DNA-chitosan nanoparticles improve DNA vaccine-elicited immunity against Newcastle disease virus through shuttling chicken interleukin-2 gene

• Published in: Journal of microencapsulation Vol. 27; no. 8; pp. 693 - 702
• Main Authors: Zhang, Wenlong, Yin, Zhe, Liu, Nihong, Yang, Tao, Wang, Junwei, Bu, Zhigao, Wu, Donglai
• Format: Journal Article
• Language: English
• Published: Colchester Informa UK, Ltd 01.12.2010; Taylor & Francis; Informa
• Subjects: Adjuvants, Immunologic; Animals; Biological and medical sciences; Cell Line; Cell Survival - drug effects; Chicken interleukin-2 gene; Chickens - physiology; Chitosan - chemistry; Chitosan - toxicity; Deoxyribonuclease I - chemistry; DNA - administration & dosage; DNA - chemistry; DNA-chitosan nanoparticles; Endocytosis - drug effects; Enzyme-Linked Immunosorbent Assay; General pharmacology; Hemagglutination Inhibition Tests; immunity; Immunization; Interferon-gamma - blood; Interleukin-2 - genetics; Medical sciences; Nanoparticles; Newcastle Disease - immunology; Newcastle Disease - prevention & control; Newcastle disease virus; Newcastle disease virus - immunology; Pharmaceutical technology. Pharmaceutical industry; Pharmacology. Drug treatments; Plasmids - genetics; Transfection; Vaccines, DNA - chemistry; Vaccines, DNA - immunology; Vaccines, DNA - toxicity; Viral Vaccines - chemistry; Viral Vaccines - immunology; Viral Vaccines - toxicity; Pharmaceutical technology; Nanoparticle; Chicken interleukin-2 gene; Polymer; Genetic vaccine; Immunity; Paramyxoviridae; DNA-chitosan nanoparticles; Virus; Polyelectrolyte; Paramyxovirinae; Mononegavirales; Gene; Interleukin 2; DNA; Rubulavirus; Genetics; Microparticle; Oside polymer; Chitosan; Newcastle disease virus; Nucleic acid
• ISSN: 0265-2048; 1464-5246
• DOI: 10.3109/02652048.2010.507881

In this study, pCAGG-ChIL2 plasmid DNA containing the chicken interleukin-2 (ChIL-2) gene was used to prepare DNA-chitosan nanoparticles (CNPs). The CNPs prepared were spherical, with mean diameters between 100 and 200 nm, have a positive surface charge, and could protect DNA against DNase I degradation. The CNPs prepared were successfully used to transfect the Df-1 cell line with almost no cytotoxicity. CNPs prepared at an amino group to phosphate group ratio (N/P ratio) of 16 provided the highest transfection efficiency (1.1%) in medium with a pH of 6.5. When pCAGG-ChIL2 CNPs were administered to chickens simultaneously with a DNA vaccine against Newcastle disease virus (NDV), haemagglutination inhibition antibody titers and serum interferon-γ (IFN-γ) levels were significantly higher than in chickens immunised with the NDV DNA vaccine alone (p < 0.05). The results demonstrate that pCAGG-ChIL2 CNPs improve DNA vaccine-elicited immunity against NDV challenge.