Egg parasitoids of the genus Trichogramma (Hymenoptera, Trichogrammatidae) in olive groves of the Mediterranean region

• Published in: Biological control Vol. 40; no. 1; pp. 48 - 56
• Main Authors: Herz, Annette, Hassan, Sherif A., Hegazi, Esmat, Khafagi, Wedad E., Nasr, Feeby N., Youssef, Ali I., Agamy, Essam, Blibech, Imen, Ksentini, Ines, Ksantini, Mohieddine, Jardak, Taieb, Bento, Albino, Pereira, José A., Torres, Laura, Souliotis, Costas, Moschos, Theodorus, Milonas, Panos
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 2007
• Subjects: Biocontrol agent; Biodiversity; biological control agents; biotypes; Ephestia kuehniella; geographical distribution; Hymenoptera; insect morphology; insect surveys; internal transcribed spacer 2; internal transcribed spacers; ITS-2 region; Mediterranean region; molecular systematics; Olea; Olea europaea; Olive cultivation; ova; Palpita unionalis; parasitoid identification; parasitoids; Prays oleae; ribosomal DNA; Sitotroga cerealella; Trichogramma; Trichogramma bourarachae; Trichogramma cacoeciae; Trichogramma cordubensis; Trichogramma euproctidis; Trichogramma nerudai; Trichogramma oleae; Trichogrammatidae; Greece; Mediterranean region; Portugal; Tunisia; Egypt; Palpita unionalis; Trichogramma cordubensis; Biodiversity; ITS-2 region; Trichogramma cacoeciae; Prays oleae; Trichogramma nerudai; Biocontrol agent; Trichogramma bourarachae; Olive cultivation; Mediterranean region; Trichogramma euproctidis; Trichogramma oleae
• ISSN: 1049-9644; 1090-2112
• DOI: 10.1016/j.biocontrol.2006.08.002

A survey of egg parasitoids of the genus Trichogramma (Hymenoptera, Trichogrammatidae) was carried out in olive groves in Portugal, Greece, Egypt, and Tunisia during the years 2002–2004. Parasitoids were obtained either by exposing sentinel eggs ( Sitotroga cerealella Olivier or Ephestia kuehniella Zeller) on olive trees or by collecting eggs of lepidopterous olive pests. Parasitized egg samples were reared separately in the laboratory for emergence of parasitoids. These were further reared in separate lines and processed by morphological and molecular biology techniques for species characterization. The recorded fauna of Trichogramma parasitoids in olive groves was species poor and consisted of species mainly known from the Mediterranean region. Trichogramma bourarachae Pintureau and Babault was found in Tunisia and Egypt, T. cordubensis Vargas and Cabello, and T. euproctidis Girault in Egypt, Trichogramma cacoeciae Marchal in Portugal, Greece, Egypt, Tunisia and Trichogramma nerudai Pintureau and Gerding in Portugal. Apart from that, Trichogramma oleae Voegelé and Pointel was collected in Tunisia. This species is probably not indigenous, but has established after several releases of a French strain were made in recent years. For selected strains, the sequence of the internal transcribed spacer 2 (ITS-2) region of rDNA was determined and deposited in the GenBank database. Differences in important biological attributes were found among collected strains of T. bourarachae, suggesting the existence of biotypes. The results contribute to the limited knowledge on distribution and biodiversity of the genus Trichogramma in the Mediterranean region. They can be helpful for the preservation and use of indigenous Trichogramma species in biological control of lepidopterous pests in olive and other local crops.